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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2468-50 | 50 µl (1.03 mg/ml) | $585 |
IHC‑plus™ Monoclonal Mouse anti‑Bovine HSPG2 / Perlecan Antibody (clone A71, IHC, WB) LS‑B2468
IHC‑plus™ Monoclonal Mouse anti‑Bovine HSPG2 / Perlecan Antibody (clone A71, IHC, WB) LS‑B2468
Note: This antibody replaces LS-C15757
Antibody:
HSPG2 / Perlecan Mouse anti-Bovine Monoclonal (A71) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP, ELISA
Reactivity:
Bovine, Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HSPG2 / Perlecan Mouse anti-Bovine Monoclonal (A71) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, IP, ELISA
Reactivity:
Bovine, Human
Format:
Unconjugated, Unmodified
Specifications
Description
Perlecan antibody LS-B2468 is an unconjugated mouse monoclonal antibody to Perlecan (HSPG2) from bovine. It is reactive with human and bovine. Validated for ELISA, IHC, IP and WB.
Target
Bovine HSPG2 / Perlecan
Synonyms
HSPG2 | Endorepellin (domain V region) | HSPG | Heparan sulfate proteoglycan 2 | Perlecan | Perlecan proteoglycan | SJA | SJS1 | PRCAN | SJS
Host
Mouse
Reactivity
Bovine, Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
A71
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Immunogen
Bovine corneal endothelial cells.
Specificity
Highly specific for bovine perlecan. The epitope is located in domain I. No cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species cross-reactivity: Human.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- IHC - Frozen
- Western blot (1:50)
- Immunoprecipitation
- ELISA (1:4000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2468 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2468 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HSPG2 / Perlecan
Validation
Anti-HSPG2 / Perlecan antibody IHC of human thymus, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HSPG2 / Perlecan antibody IHC of human thymus, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ HSPG2 / Perlecan Antibody (clone A71) for IHC, WB/Western, IP, ELISA LS-B2468 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Human, Mouse, Rat, Bovine
Applications:
IHC, IHC - Frozen, ICC, Western blot, Immunoprecipitation
Species:
Bovine, Human
Applications:
IHC, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Species:
Bovine, Human
Applications:
IHC, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Species:
Mouse, Human, Monkey, Bovine, Pig
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Reactivity:
Human
Range:
0.624-40 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
