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Anti-HSPG2 / Perlecan Antibody (clone A71) IHC-plus™ LS-B2468

Note: This antibody replaces LS-C15757

Ordering

Wt. Vol. Conc. Price
- 50 µl 1.03 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HSPG2 / Perlecan Antibodies

Anti-HSPG2 / Perlecan Antibody (clone A7L6) LS-C15761
Rat Monoclonal [clone A7L6] (IgG2a,k) to Mouse HSPG2 / Perlecan
Mouse, Human, Bovine, Pig
IHC - Frozen, Immunofluorescence
Unconjugated
Anti-HSPG2 / Perlecan Antibody (clone A71) IHC-plus™ LS-B2468
Mouse Monoclonal [clone A71] (IgG1,k) to Bovine HSPG2 / Perlecan
Bovine, Human
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone A71] (IgG1,k) to Bovine HSPG2 / Perlecan
Bovine, Human
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Bovine HSPG2 / Perlecan
Mouse
Bovine, Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [A71]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • IHC - Frozen
  • Western blot (1:50)
  • Immunoprecipitation
  • ELISA (1:4000)

Specificity and Use

HSPG2 / Perlecan antibody was raised against bovine corneal endothelial cells.
Highly specific for bovine perlecan. The epitope is located in domain I. No cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). Species cross-reactivity: Human.
Immunohistochemistry: LS-B2468 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2468 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 15mM sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HSPG2 / Perlecan

P98160 NM_005529 NP_005520.4

HSPG2 Antibody, Endorepellin (domain V region) Antibody, HSPG Antibody, Heparan sulfate proteoglycan 2 Antibody, Perlecan Antibody, Perlecan proteoglycan Antibody, SJA Antibody, SJS1 Antibody, PRCAN Antibody, SJS Antibody

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury.

Immunohistochemistry

Immunohistochemistry
Anti-HSPG2 / Perlecan antibody IHC of human thymus, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2468 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/3/2016

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