Human HSPD1 / HSP60

Mouse

Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Chicken, C. elegans, Plant (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

LK-2

Unconjugated

Protein G purified

Unmodified

Human HSP60 produced through recombinant DNA methods in E. coli.

Detects an ~60 kD protein, corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, chicken, dog, guinea pig, hamster, monkey, pig, rabbit, plant (spinach), Borrelia, E. coli, helicobacter pylori, M. bovis (Hsp65), Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Yersinia entercolitica, and yeast origins. Deletion mutant analysis indicates that the epitope recognized by LK-2 is located between residues 383-419 of the human Hsp60 sequence (which corresponds to residues 356-393 of the mycobacterial Hsp60 sequence).

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (0.25 µg/ml)
• Immunoprecipitation (10 µg/ml)

Immunohistochemistry: LS-B3678 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3678 was determined to be 10 ug/ml.

PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P10809 NCBI: NM_002156 NP_002147.2