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Anti-HSPD1 / HSP60 Antibody (clone LK-2) IHC-plus™ LS-B3678

Note: This antibody replaces LS-C63244

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HSPD1 / HSP60 Antibodies

Anti-HSPD1 / HSP60 Antibody (aa150-200) IHC-plus™ LS-B2040
Rabbit Polyclonal to Human HSPD1 / HSP60
Human, Monkey, Bovine, Dog
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HSPD1 / HSP60 Antibody LS-C109067
Rabbit Polyclonal to Plasmodium falciparum HSPD1 / HSP60
Plasmodium falciparum
Immunofluorescence, Western blot
Unconjugated
Western blot Image
Anti-HSPD1 / HSP60 Antibody (Internal, Magnetic beads) LS-C171866
Rabbit Polyclonal to Human HSPD1 / HSP60
Human, Mouse, Rat
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Immunoprecipitation
Magnetic beads Conjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone LK-2] (IgG1) to Human HSPD1 / HSP60
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Chicken, C. elegans, Plant
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human HSPD1 / HSP60
Mouse
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Chicken, C. elegans, Plant (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [LK-2]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.25 µg/ml)
  • Immunoprecipitation (10 µg/ml)

Specificity and Use

HSPD1 / HSP60 antibody was raised against human HSP60 produced through recombinant DNA methods in E. coli.
Detects an ~60 kD protein, corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, chicken, dog, guinea pig, hamster, monkey, pig, rabbit, plant (spinach), Borrelia, E. coli, helicobacter pylori, M. bovis (Hsp65), Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Yersinia entercolitica, and yeast origins. Deletion mutant analysis indicates that the epitope recognized by LK-2 is located between residues 383-419 of the human Hsp60 sequence (which corresponds to residues 356-393 of the mycobacterial Hsp60 sequence).
Immunohistochemistry: LS-B3678 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3678 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.2, 0.1 mM PMSF, 50% glycerol
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
For research use only.

About HSPD1 / HSP60

P10809 NM_002156 NP_002147.2

HSPD1 Antibody, 60 kDa chaperonin Antibody, CPN60 Antibody, Heat shock protein 65 Antibody, Heat shock protein 60 Antibody, HSP-60 Antibody, HSP60 Antibody, HuCHA60 Antibody, HLD4 Antibody, HSP65 Antibody, p60 lymphocyte protein Antibody, SPG13 Antibody, Chaperonin 60 Antibody, GROEL Antibody

HSPD1 / HSP60 is a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene.

Immunohistochemistry

Immunohistochemistry
Anti-HSPD1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3678 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-HSPD1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3678 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-HSPD1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3678 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/9/2016

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