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Catalog Number Size Price
LS-C357530-10 10 µg $318 
LS-C357530-100 100 µg (0.5 mg/ml) $470 
HSPD1 / HSP60 Antibody - Hsp60 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
HSPD1 / HSP60 Antibody - Hsp60 antibody IHC-paraffin: Rat Intestine Tissue.
HSPD1 / HSP60 Antibody - Hsp60 antibody IHC-paraffin: Mouse Intestine Tissue.
HSPD1 / HSP60 Antibody - Hsp60 antibody ICC: SMMC-7721 Cell.
HSPD1 / HSP60 Antibody - IF analysis of Hsp60 using anti-Hsp60 antibody Hsp60 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Hsp60 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HSPD1 / HSP60 Antibody - Hsp60 antibody Western blot. All lanes: Anti Hsp60 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: Mouse Kidney Tissue Lysate at 50 ug. Lane 3: HEPA Whole Cell Lysate at 40 ug. Lane 4: NRK Whole Cell Lysate at 40 ug. Lane 5: PC-12 Whole Cell Lysate at 40 ug. Lane 6: SW620 Whole Cell Lysate at 40 ug. Lane 7: A549 Whole Cell Lysate at 40 ug. Lane 8: A431 Whole Cell Lysate at 40 ug. Lane 9: HELA Whole Cell Lysate at 40 ug. Predicted band size: 60 kD. Observed band size: 60 kD.
HSPD1 / HSP60 Antibody - Flow Cytometry analysis of A431 cells using anti-Hsp60 antibody. Overlay histogram showing A431 cells stained with anti-Hsp60 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsp60 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
HSPD1 / HSP60 Antibody - Hsp60 antibody IHC-paraffin: Human Intestinal Cancer Tissue.
HSPD1 / HSP60 Antibody - Hsp60 antibody IHC-paraffin: Rat Intestine Tissue.
HSPD1 / HSP60 Antibody - Hsp60 antibody IHC-paraffin: Mouse Intestine Tissue.
HSPD1 / HSP60 Antibody - Hsp60 antibody ICC: SMMC-7721 Cell.
HSPD1 / HSP60 Antibody - IF analysis of Hsp60 using anti-Hsp60 antibody Hsp60 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Hsp60 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HSPD1 / HSP60 Antibody - Hsp60 antibody Western blot. All lanes: Anti Hsp60 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: Mouse Kidney Tissue Lysate at 50 ug. Lane 3: HEPA Whole Cell Lysate at 40 ug. Lane 4: NRK Whole Cell Lysate at 40 ug. Lane 5: PC-12 Whole Cell Lysate at 40 ug. Lane 6: SW620 Whole Cell Lysate at 40 ug. Lane 7: A549 Whole Cell Lysate at 40 ug. Lane 8: A431 Whole Cell Lysate at 40 ug. Lane 9: HELA Whole Cell Lysate at 40 ug. Predicted band size: 60 kD. Observed band size: 60 kD.
HSPD1 / HSP60 Antibody - Flow Cytometry analysis of A431 cells using anti-Hsp60 antibody. Overlay histogram showing A431 cells stained with anti-Hsp60 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsp60 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human HSPD1 / HSP60 Antibody (aa260‑496, IHC, WB) LS‑C357530

Polyclonal Rabbit anti‑Human HSPD1 / HSP60 Antibody (aa260‑496, IHC, WB) LS‑C357530

Note: This antibody replaces LS-C388616
Antibody:
HSPD1 / HSP60 Rabbit anti-Human Polyclonal (aa260-496) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357530-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPD1 / HSP60 Rabbit anti-Human Polyclonal (aa260-496) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HSP60 antibody LS-C357530 is an unconjugated rabbit polyclonal antibody to HSP60 (HSPD1) (aa260-496) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB.
Target
Human HSPD1 / HSP60
Synonyms
HSPD1 | 60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Hsp60 recombinant protein (Position: A260-Q496). Human Hsp60 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp60.
Epitope
aa260-496
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPD1 / HSP60
P10809 NM_002156 NP_002147.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024