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Antibodies > HSPD1 / HSP60 > LS-C97358
 
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Catalog Number Size Price
LS-C97358-400 400 µl $393 
HSPD1 / HSP60 Antibody - Fluorescent confocal image of HeLa cell stained with HSPD1 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HSPD1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HSPD1 immunoreactivity is localized to Mitochondria significantly.
HSPD1 / HSP60 Antibody - Fluorescent image of U251 cells stained with HSPD1 antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated HSPD1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Note the highly specific localization of the HSPD1 mainly to the mitochondria, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000144381).
HSPD1 / HSP60 Antibody - HSPD1 Antibody western blot of A431 cell line lysates (35 ug/lane). The HSPD1 antibody detected the HSPD1 protein (arrow).
HSPD1 / HSP60 Antibody - Fluorescent confocal image of HeLa cell stained with HSPD1 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HSPD1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HSPD1 immunoreactivity is localized to Mitochondria significantly.
HSPD1 / HSP60 Antibody - Fluorescent image of U251 cells stained with HSPD1 antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated HSPD1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Note the highly specific localization of the HSPD1 mainly to the mitochondria, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000144381).
HSPD1 / HSP60 Antibody - HSPD1 Antibody western blot of A431 cell line lysates (35 ug/lane). The HSPD1 antibody detected the HSPD1 protein (arrow).
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Polyclonal Rabbit anti‑Human HSPD1 / HSP60 Antibody (aa187‑215, IF, WB) LS‑C97358

Polyclonal Rabbit anti‑Human HSPD1 / HSP60 Antibody (aa187‑215, IF, WB) LS‑C97358

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Antibody:
HSPD1 / HSP60 Rabbit anti-Human Polyclonal (aa187-215) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C97358-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HSPD1 / HSP60 Rabbit anti-Human Polyclonal (aa187-215) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HSP60 antibody LS-C97358 is an unconjugated rabbit polyclonal antibody to human HSP60 (HSPD1) (aa187-215). Validated for IF and WB.
Target
Human HSPD1 / HSP60
Synonyms
HSPD1 | 60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Hamster, Chicken, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa187-215
Specificity
This HSPD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-215 amino acids from the Central region of human HSPD1.
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPD1 / HSP60
P10809 NM_002156 NP_002147.2

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PathPlus™ HSPD1 / HSP60 Antibody LS‑B3679
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HSPD1 / HSP60 Antibody - This image was taken for the unconjugated form of this product. Other forms have not been tested.
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See all HSPD1 / HSP60 Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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