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Anti-HSPB1 / HSP27 Antibody (phospho-Ser78) LS-C97053

Catalog	Size	Price
LS-C97053-400	400 µl	Unavailable

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Antibody:	Mouse Monoclonal [clone 5D12-A12] (IgG2b,k) to Human HSPB1 / HSP27
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Anti-HSPB1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7450 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Anti-HSPB1 / HSP27 Antibody (clone SJ-25) LS-C171154

Antibody:	Mouse Monoclonal [clone SJ-25] (IgG1) to Turkey HSPB1 / HSP27
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Antibody:	Rabbit Polyclonal to Human HSPB1 / HSP27
Reactivity:	Human
Application:	Dot Blot
Format:	Unconjugated

Dot blot of Phospho-HSBP1-S78 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-antibody: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.

Details

Target:	Human HSPB1 / HSP27
Host:	Rabbit
Reactivity:	Human (tested or 100% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated
Purification:	Protein A purified
Modifications:	Unmodified

Applications

Approved:

Dot Blot (1:500)

Specificity and Use

Blocking Peptide:

LS-E7354 - Lyophilized - 100 µg - $230.00
Immunizing peptide used to generate LS-C97053. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

pSer78

Specificity:

This HSPB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S78 of human HSPB1.

Packaging

Presentation:	PBS, 0.09% sodium azide
Storage:	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions:	For research use only.

About HSPB1 / HSP27

Uniprot: P04792 NCBI: NM_001540 NP_001531.1

Synonyms: HSPB1 Antibody, 28 kDa heat shock protein Antibody, Heat shock 27 kDa protein Antibody, Heat shock 27kD protein 1 Antibody, Heat shock 27kDa protein 1 Antibody, HSP27 Antibody, HSP28 Antibody, HSP 27 Antibody, Hsp25 Antibody, SRP27 Antibody, Stress-responsive protein 27 Antibody, CMT2F Antibody, Heat shock 27 kd protein Antibody, Heat shock protein beta-1 Antibody, HMN2B Antibody, HS.76067 Antibody

Description: Heat shock protein 27 (Hsp27) also known as heat shock protein beta-1 (HSPB1) is a protein that in humans is encoded by the HSPB1 gene. Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, a-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction.

(More About HSPB1 / HSP27)

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Technical Help Review

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Dot Blot

Dot blot of Phospho-HSBP1-S78 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-antibody: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.

Requested From:
Date Requested: 4/23/2018

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