Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
HSPB1 / HSP27 antibody was raised against a 12 residue synthetic peptide, YSRAL[pS]RQLSS(C) corresponding to amino acids 73-83 of human Hsp27 with the cysteine (C) residue added and the peptide coupled to KLH.
This affinity purified antibody detects an ~27kD protein, corresponding to the apparent molecular mass of phosphorylated Hsp27 (Ser78) on SDS-PAGE immunoblots, in samples from human, monkey (weak), bovine and guinea pig. This antibody is specific for phosphorylated Hsp27 (Ser78) and does not exhibit reactivity with non-phosphorylated Hsp27.
Suitable for use in Western Blot, Immunoprecipitation. Western Blot (Colorimetric): 1:2000; 1:1000 (ECL). Immunoprecipitation: 1:100.
PBS, pH 7.2, 50% glycerol, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
HSPB1 Antibody, 28 kDa heat shock protein Antibody, Heat shock 27 kDa protein Antibody, Heat shock 27kD protein 1 Antibody, Heat shock 27kDa protein 1 Antibody, HSP27 Antibody, HSP28 Antibody, HSP 27 Antibody, Hsp25 Antibody, SRP27 Antibody, Stress-responsive protein 27 Antibody, CMT2F Antibody, Heat shock 27 kd protein Antibody, Heat shock protein beta-1 Antibody, HMN2B Antibody, HS.76067 Antibody
Heat shock protein 27 (Hsp27) also known as heat shock protein beta-1 (HSPB1) is a protein that in humans is encoded by the HSPB1 gene. Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, a-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction.