Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000)
Western blot (1:500 - 1:3000)
Specificity and Use
HSPB1 / HSP27 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 205 of HSP27 (SwissProt P04792). Percent identity by BLAST analysis:
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000, IP: 1:500-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
HSPB1 Antibody, 28 kDa heat shock protein Antibody, Heat shock 27 kDa protein Antibody, Heat shock 27kD protein 1 Antibody, Heat shock 27kDa protein 1 Antibody, HSP27 Antibody, HSP28 Antibody, HSP 27 Antibody, Hsp25 Antibody, SRP27 Antibody, Stress-responsive protein 27 Antibody, CMT2F Antibody, Heat shock 27 kd protein Antibody, Heat shock protein beta-1 Antibody, HMN2B Antibody, HS.76067 Antibody
Heat shock protein 27 (Hsp27) also known as heat shock protein beta-1 (HSPB1) is a protein that in humans is encoded by the HSPB1 gene. Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, a-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction.
IHC of paraffin-embedded U87 xenograft, using HSP27 antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 12% SDS PAGE HSPB1 antibody diluted at 1:1000
HSP27 antibody immunoprecipitates HSP27 protein in IP experiments. IP Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of HSP27 protein by 2.5 ug of HSP27 15% SDS-PAGE The immunoprecipitated HSP27 protein was detected by HSP27 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Requested From: United States
Date Requested: 2/26/2017