Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Protein A purified
IHC - Paraffin
IHC - Frozen
Western blot (1:500 - 1:1000)
Specificity and Use
HSPB1 / HSP27 antibody was raised against prokaryotic recombinant protein corresponding to the full length human hsp27 protein.
Recognizes HSP27 in human tissues and cell lines at 27 kD. Does not cross react with HSP70, HSP90 or HSP104.
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:500-1:1000. Immunohistochemistry (Frozen and paraffin): 1:50. Immunocytochemistry: Frozen and paraffin. Positive control: MCF-7 cells.
PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
HSPB1 Antibody, 28 kDa heat shock protein Antibody, Heat shock 27 kDa protein Antibody, Heat shock 27kD protein 1 Antibody, Heat shock 27kDa protein 1 Antibody, HSP27 Antibody, HSP28 Antibody, HSP 27 Antibody, Hsp25 Antibody, SRP27 Antibody, Stress-responsive protein 27 Antibody, CMT2F Antibody, Heat shock 27 kd protein Antibody, Heat shock protein beta-1 Antibody, HMN2B Antibody, HS.76067 Antibody
Heat shock protein 27 (Hsp27) also known as heat shock protein beta-1 (HSPB1) is a protein that in humans is encoded by the HSPB1 gene. Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, a-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction.