Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, ICC, Western blot, Immunoprecipitation, ELISA
Human HSPB1 / HSP27
Human (tested or 100% immunogen sequence identity)
Protein A purified
IHC - Paraffin (1.25 µg/ml)
Western blot (1:1000)
Specificity and Use
HSPB1 / HSP27 antibody was raised against recombinant human HSPB1.
Prokaryotic the full length human hsp27 protein.
Immunohistochemistry: LS-B383 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B383 was determined to be 1.25 ug/ml.
0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
Chromosomal assignments of human 27-kDa heat shock protein gene family. McGuire SE, Fuqua SA, Naylor SL, Helin-Davis DA, McGuire WL. Somatic cell and molecular genetics. 1989 15:167-71.
A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells. Moretti-Rojas I, Fuqua SA, Montgomery RA, McGuire WL. Breast cancer research and treatment. 1988 11:155-63.
Estradiol stimulates synthesis of a major intracellular protein in a human breast cancer cell line (MCF-7). Edwards DP, Adams DJ, McGuire WL. Breast cancer research and treatment. 1981 1:209-23.
HSPB1 Antibody, 28 kDa heat shock protein Antibody, Heat shock 27 kDa protein Antibody, Heat shock 27kD protein 1 Antibody, Heat shock 27kDa protein 1 Antibody, HSP27 Antibody, HSP28 Antibody, HSP 27 Antibody, Hsp25 Antibody, SRP27 Antibody, Stress-responsive protein 27 Antibody, CMT2F Antibody, Heat shock 27 kd protein Antibody, Heat shock protein beta-1 Antibody, HMN2B Antibody, HS.76067 Antibody
Heat shock protein 27 (Hsp27) also known as heat shock protein beta-1 (HSPB1) is a protein that in humans is encoded by the HSPB1 gene. Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, a-crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction.
Anti-HSPB1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B383 concentration 5 ug/ml.
Anti-Human HSP27 Monoclonal Antibody - Western Blot. Mab anti-Human HSP27 antibody (clone G3.1) is shown to detect human HSP27 by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1000 dilution of Mab anti-Human HSP27 at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] ( diluted 1:5000. A single band corresponding to human HSP27 is detected at ~27 kD when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human HSP27. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Mab anti-Human HSP27 antibody (clone G3.1) is shown to detect human HSP27 by western blot. Detection occurs after 10 mg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human HSP27.