Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Locations
Orders Processing,
Shipping & Receiving,
Warehouse
2 Shaker Rd Suites
B001/B101
Shirley, MA 01464
Production Lab
Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036
Telephone Numbers
Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-B7450-50 | 50 µg (1 mg/ml) | $460 |
1 of 2
2 of 2
IHC‑plus™ Monoclonal Mouse anti‑Human HSPB1 / HSP27 Antibody (clone 5D12‑A12, IHC, WB) LS‑B7450
IHC‑plus™ Monoclonal Mouse anti‑Human HSPB1 / HSP27 Antibody (clone 5D12‑A12, IHC, WB) LS‑B7450
Note: This antibody replaces LS-C108985
Antibody:
HSPB1 / HSP27 Mouse anti-Human Monoclonal (5D12-A12) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HSPB1 / HSP27 Mouse anti-Human Monoclonal (5D12-A12) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
HSP27 antibody LS-B7450 is an unconjugated mouse monoclonal antibody to human HSP27 (HSPB1). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HSPB1 / HSP27
Synonyms
HSPB1 | 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Clone
5D12-A12
Conjugations
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Immunogen
Human Hsp27.
Specificity
Detects a 27kD protein corresponding to the molecular mass of hsp27 on SDS PAGE immunoblots. There is no cross-reactivity to alphaBcrystallin.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (0.25 - 0.5 µg/ml)
- Immunoprecipitation
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HSPB1 / HSP27
Validation
Anti-HSPB1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HSPB1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ HSPB1 / HSP27 Antibody (clone 5D12-A12) for IHC, WB/Western, IP, ELISA LS-B7450 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Turkey, Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
Species:
Human, Chimpanzee, Mouse, Rat, Sheep, Chicken
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Species:
Human, Monkey, Bovine, Guinea pig, Pig
Applications:
Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/15/2024
Date Requested: 4/15/2024
