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Anti-HSPA5 / GRP78 / BiP Antibody (aa550-600) IHC-plus™ LS-B2038

Note: This antibody replaces LS-C637

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HSPA5 / GRP78 / BiP Antibodies

Anti-HSPA5 / GRP78 / BiP Antibody IHC-plus™ LS-B3388
Rabbit Polyclonal to Human HSPA5 / GRP78 / BiP
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-HSPA5 / GRP78 / BiP Antibody (aa541-568) LS-C165063
Rabbit Polyclonal to Human HSPA5 / GRP78 / BiP
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-HSPA5 / GRP78 / BiP Antibody (Gly584) IHC-plus™ LS-B9789
Rabbit Monoclonal (IgG) to Human HSPA5 / GRP78 / BiP
Human, Mouse
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal (IgG) to Human HSPA5 / GRP78 / BiP
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human HSPA5 / GRP78 / BiP
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 3 µg/ml)

Specificity and Use

HSPA5 / GRP78 / BiP antibody was raised against a portion of amino acids 550-600 of human GRP78.
aa550-600
A portion of amino acids 550-600 of human BiP was used as the immunogen.
Immunohistochemistry: LS-B2038 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2038 was determined to be 10 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About HSPA5 / GRP78 / BiP

P11021 M19645 AAA52614.1

HSPA5 Antibody, BIP Antibody, Heat shock 70 kDa protein 5 Antibody, MIF2 Antibody, GRP-78 Antibody, GRP78 Antibody

Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

Immunohistochemistry

Immunohistochemistry
Anti-HSPA5 / GRP78 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2038 concentration 10 ug/ml.

Western blot

Western blot
Western blot of GRP78 in mouse liver tissue enriched for endoplasmic reticulum, and 1 ug/ml of LS-B2038 (anti-GRP78) and 0.25 ug/ml of anti-GAPDH loading control. Lane A contains 20 ugs of whole mouse liver lysate, lane B contains 20 ugs of total ER fraction, and lane C contains 20 ugs of rough ER fraction. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Western blot

Western blot
Western blot of BiP in HeLa cell lysate using antibody at 1 ug/ml.
 

Requested From: United States
Date Requested: 12/9/2016

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