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Anti-HSP90B1 / GP96 / GRP94 Antibody (aa676-803, clone 2H3) IHC-plus™ LS-B2929

Note: This antibody replaces LS-C93799

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HSP90B1 / GP96 / GRP94 Antibodies

Anti-HSP90B1 / GP96 / GRP94 Antibody (N-Terminus) LS-C15352
Rabbit Polyclonal to Human HSP90B1 / GP96 / GRP94
Human, Mouse, Rat
Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-HSP90B1 / GP96 / GRP94 Antibody (aa676-803, clone 2H3) IHC-plus™ LS-B2929
Mouse Monoclonal [clone 2H3] (IgG2a,k) to Human HSP90B1 / GP96 / GRP94
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HSP90B1 / GP96 / GRP94 Antibody (aa593-606) IHC-plus™ LS-B3243
Goat Polyclonal to Human HSP90B1 / GP96 / GRP94
Human, Monkey, Mouse, Bat, Dog, Hamster, Horse, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2H3] (IgG2a,k) to Human HSP90B1 / GP96 / GRP94
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human HSP90B1 / GP96 / GRP94
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [2H3]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1:100 - 1:200)
  • Western blot
  • ELISA

Specificity and Use

HSP90B1 / GP96 / GRP94 antibody was raised against recombinant human TRA1 (676-803aa) purified from E. coli.
aa676-803
Immunohistochemistry: LS-B2929 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2929 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About HSP90B1 / GP96 / GRP94

P14625 NM_003299 NP_003290.1

HSP90B1 Antibody, ECGP Antibody, Endoplasmin Antibody, gp96 homolog Antibody, gp96 Antibody, GRP-94 Antibody, GRP94 Antibody, Tumor rejection antigen 1 Antibody, TRA1 Antibody

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.

Immunohistochemistry

Immunohistochemistry
Anti-HSP90B1 / GRP94 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2929 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-HSP90B1 / GRP94 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2929 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-HSP90B1 / GRP94 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2929 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3).

Western blot

Western blot
The cell lysates of hES(20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
 

Requested From: United States
Date Requested: 12/6/2016

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