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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSP90 Beta Antibody (phospho‑Ser254) LS‑C199088
HSP90 Beta antibody LS-C199088 is an unconjugated rabbit polyclonal antibody to HSP90 Beta from human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C199088-50
50 µl
Unavailable
LS-C199088-100
100 µl
Unavailable

Popular HSP90 Beta Products

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Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
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Western blot of HSP90B phosphorylation expression in TNF-a treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Species: Human, Mouse, Rat
Applications: IHC, ICC, Immunofluorescence, Western blot
Western blot of HSP90B expression in TNF-a treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Species: Human, Mouse, Rat
Applications: IHC, ICC, Immunofluorescence, Western blot

Product Description

HSP90 Beta antibody LS-C199088 is an unconjugated rabbit polyclonal antibody to HSP90 Beta from human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
About HSP90 Beta
In mammalian cells, there are two or more genes encoding cytosolic Hsp90 homologues, with the human Hsp90 alpha showing 85% sequence identity to Hsp90 beta. The alpha- and the beta-forms are thought to be the result of a gene duplication event that occurred millions of years ago. HSP90 Beta is encoded by the HSP90B1 gene and is located in the endoplasmic reticulum.

Specifications

Target
Human HSP90 Beta
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HSP90 Beta antibody was raised against synthetic peptide derived from human HSP90B around the phosphorylation site of Ser254.
Epitope
pSer254
Specificity
HSP90B (Phospho-Ser254) Antibody detects endogenous levels of HSP90B only when phosphorylated at serine254.
Presentation
PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.

Immunocytochemistry

Immunofluorescence of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.

Immunocytochemistry

Immunofluorescence of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.

Immunocytochemistry

Immunofluorescence of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right was incubated with synthetic peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.

Immunocytochemistry

Immunofluorescence of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.
Immunofluorescence of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right was incubated with synthetic peptide.


Requested From: 
Date Requested: 6/18/2018

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