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Anti-HSP90 / Heat Shock Protein 90 Antibody (aa1-732, clone 4F10) IHC-plus™ LS-B2934

Note: This antibody replaces LS-C93748

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HSP90 / Heat Shock Protein 90 Antibodies

Anti-HSP90 / Heat Shock Protein 90 Antibody (clone D7A) LS-C108967
Mouse Monoclonal [clone D7A] (IgG) to Chicken HSP90 / Heat Shock Protein 90
Chicken, Human, Mouse, Rat, Bovine, Pig, Rabbit
IHC, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HSP90 / Heat Shock Protein 90 Antibody (aa676-693, Magnetic beads) LS-C172172
Rabbit Polyclonal to Human HSP90 / Heat Shock Protein 90
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation
Magnetic beads Conjugated
Immunohistochemistry Image
Anti-HSP90 / Heat Shock Protein 90 Antibody (Acetyl-Lys435) LS-C380587
Rabbit Polyclonal (IgG) to Human HSP90 / Heat Shock Protein 90
Human, Mouse, Rat
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4F10] (IgG2b,k) to Human HSP90 / Heat Shock Protein 90
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human HSP90 / Heat Shock Protein 90
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [4F10]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000 - 1:2000)
  • ELISA

Specificity and Use

Recombinant human Hsp90 (1-732aa) purified from E. coli.
aa1-732
Immunohistochemistry: LS-B2934 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2934 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About HSP90 / Heat Shock Protein 90

Hsp90 (heat shock protein 90) is a chaperone protein that assists other proteins to fold properly, stabilizes proteins against heat stress, and aids in protein degradation. It also stabilizes a number of proteins required for tumor growth, which is why Hsp90 inhibitors are investigated as anti-cancer drugs. Heat shock proteins, as a class, are among the most highly expressed cellular proteins across all species.

Immunohistochemistry

Immunohistochemistry
Anti-HSP90 / Heat Shock Protein 90 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2934 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-HSP90 / Heat Shock Protein 90 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2934 concentration 5 ug/ml.

Western blot

Western blot
Cell lysates of HeLa (each 20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp90(1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.
 

Requested From: United States
Date Requested: 12/5/2016

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