Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Yeast (tested or 100% immunogen sequence identity)
Western blot (0.02 - 0.06 µg/ml)
Specificity and Use
HSP31 / Hsp31p antibody was raised against synthetic peptide C-ETGKFGWDEHSLAKD from an internal region of yeast HSP31 / Hsp31p (NP_010822.1). Percent identity by BLAST analysis: Yeast (100%).
LS-E27900 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C186515. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Yeast HSP31 / Hsp31p
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 24kD band observed in lysates of postdiauxic (overnight grown yeast cells, and no signal in KO grown under identical conditions. Cells grown in logarithmic phase show diminished signals as expected. (calculated MW of 25.7kD according to NP_010822.1). Recommended concentration: 0.02-0.06 ug/ml.
HSP31 antibody (0.05 ug/ml) staining of yeast lysate (wt) and the KO (delta) in the left panel and the Ponceau stain in the right panel (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained by Dr. M. Skoneczny, IBB, Warsaw, Poland.