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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSF1 Antibody (phospho‑Ser303) LS‑C117376
Note: This antibody replaces LS-C67318, LS-C51429, LS-C34206
HSF1 antibody LS-C117376 is an unconjugated rabbit polyclonal antibody to HSF1 from human and mouse. Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C117376-50
50 µl
Unavailable
LS-C117376-100
100 µl
Unavailable

Popular HSF1 Products

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Applications: IHC, IHC - Paraffin, Western blot
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Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA

Product Description

HSF1 antibody LS-C117376 is an unconjugated rabbit polyclonal antibody to HSF1 from human and mouse. Validated for IHC, Peptide-ELISA and WB.
About HSF1
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Q00613 NM_005526 NP_005517.1

HSF1 Antibody, HSTF 1 Antibody, Heat shock factor protein 1 Antibody, HSF 1 Antibody, HSTF1 Antibody


Specifications

Target
Human HSF1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HSF1 antibody was raised against synthetic peptide from human HSF1 around the phosphorylation site of Ser303.
Epitope
pSer303
Specificity
HSF1 (Phospho-Ser303) Antibody detects endogenous levels of HSF1 only when phosphorylated at serine303.
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Storage
Store at -20°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from MCF7 cells treated with TNF-alpha 20 ng/ml 30', using HSF1 (Phospho-Ser303) Antibody. The lane on the left is treated with the synthesized peptide.
Western blot of extracts from MCF7 cells treated with TNF-alpha 20 ng/ml 30', using HSF1 (Phospho-Ser303) Antibody. The lane on the left is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from MCF7 cells treated with TNF-alpha 20 ng/ml 30', using HSF1 (Phospho-Ser303) Antibody. The lane on the left is treated with the synthesized peptide.
Western blot of extracts from MCF7 cells treated with TNF-alpha 20 ng/ml 30', using HSF1 (Phospho-Ser303) Antibody. The lane on the left is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from MCF7 cells treated with TNF-alpha 20 ng/ml 30', using HSF1 (Phospho-Ser303) Antibody. The lane on the left is treated with the synthesized peptide.
Western blot of extracts from MCF7 cells treated with TNF-alpha 20 ng/ml 30', using HSF1 (Phospho-Ser303) Antibody. The lane on the left is treated with the synthesized peptide.

Immunohistochemistry

IHC of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is treated with the synthesized peptide.
IHC of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot

Western blot of extracts from MCF7 cells treated with TNF-alpha 20 ng/ml 30', using HSF1 (Phospho-Ser303) Antibody. The lane on the left is treated with the synthesized peptide.
Western blot of extracts from MCF7 cells treated with TNF-alpha 20 ng/ml 30', using HSF1 (Phospho-Ser303) Antibody. The lane on the left is treated with the synthesized peptide.


Requested From: 
Date Requested: 6/19/2018

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