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Anti-HSF1 Antibody LS-C7379

Ordering

Wt. Vol. Conc. Price
50 µg - - $395
200 µg - - $515
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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Rat Monoclonal (IgG1) to Mouse HSF1
Mouse, Human, Monkey, Rat, Bovine, Guinea pig, Hamster, Pig, Rabbit
ICC, Western blot, Immunoprecipitation, ELISA, Gel shift
Unconjugated

Details

Mouse HSF1
Rat
Mouse, Human, Monkey, Rat, Bovine, Guinea pig, Hamster, Pig, Rabbit (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Protein G purified

Applications

ICC (1:100), Western blot, Immunoprecipitation (12.5 µg/ml), ELISA, Gel shift

Specificity and Use

HSF1 antibody was raised against purified recombinant mouse HSF-1 protein.
This affinity purified antibody Detects a ~85kD protein in unstressed cell lysates and an ~95kD protein in heat shocked cell lysates, corresponding to the apparent molecular masses of the inactive and active forms of HSF1, respectively, on SDS-PAGE Western Blots, in samples from human, mouse, rat, bovine, guinea pig, hamster, monkey and rabbit.
Western Blot (ECL): 2 ug/mL. Immunoprecipitation: 12.5 ug/mL. Immunocytochemistry: 1:100. Gel Mobility Shift Assay. ELISA. Positive control: Recombinant Human HSF-1 Protein.

Packaging

PBS, pH 7.2, 0.1 mM PMSF in 50% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HSF1

Q00613 NM_005526 NP_005517.1

HSF1 Antibody, HSTF 1 Antibody, Heat shock factor protein 1 Antibody, HSF 1 Antibody, HSTF1 Antibody

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Requested From: United States
Date Requested: 9/30/2016

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