HSF1 antibody LS-C7379 is an unconjugated rat monoclonal antibody to HSF1 from human, mouse, rat and other species. Validated for ELISA, GS, ICC, IP and WB.
Mouse, Human, Monkey, Rat, Bovine, Guinea pig, Hamster, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Protein G purified
- ICC (1:100)
- Western blot
- Immunoprecipitation (12.5 µg/ml)
- Gel shift
HSF1 antibody was raised against purified recombinant mouse HSF-1 protein.
This affinity purified antibody Detects a ~85kD protein in unstressed cell lysates and an ~95kD protein in heat shocked cell lysates, corresponding to the apparent molecular masses of the inactive and active forms of HSF1, respectively, on SDS-PAGE Western Blots, in samples from human, mouse, rat, bovine, guinea pig, hamster, monkey and rabbit.
Western Blot (ECL): 2 ug/mL. Immunoprecipitation: 12.5 ug/mL. Immunocytochemistry: 1:100. Gel Mobility Shift Assay. ELISA. Positive control: Recombinant Human HSF-1 Protein.
PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.