Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse, Human, Monkey, Rat, Bovine, Guinea pig, Hamster, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Protein G purified
Immunoprecipitation (12.5 µg/ml)
HSF1 antibody was raised against purified recombinant mouse HSF-1 protein.
This affinity purified antibody Detects a ~85kD protein in unstressed cell lysates and an ~95kD protein in heat shocked cell lysates, corresponding to the apparent molecular masses of the inactive and active forms of HSF1, respectively, on SDS-PAGE Western Blots, in samples from human, mouse, rat, bovine, guinea pig, hamster, monkey and rabbit.
Western Blot (ECL): 2 ug/mL. Immunoprecipitation: 12.5 ug/mL. Immunocytochemistry: 1:100. Gel Mobility Shift Assay. ELISA. Positive control: Recombinant Human HSF-1 Protein.
PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.