Mouse, Human, Monkey, Rat, Bovine, Guinea pig, Hamster, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Protein G purified
- ICC (1:100)
- Western blot
- Immunoprecipitation (12.5 µg/ml)
- Gel shift
HSF1 antibody was raised against purified recombinant mouse HSF-1 protein.
This affinity purified antibody Detects a ~85kD protein in unstressed cell lysates and an ~95kD protein in heat shocked cell lysates, corresponding to the apparent molecular masses of the inactive and active forms of HSF1, respectively, on SDS-PAGE Western Blots, in samples from human, mouse, rat, bovine, guinea pig, hamster, monkey and rabbit.
Western Blot (ECL): 2 ug/mL. Immunoprecipitation: 12.5 ug/mL. Immunocytochemistry: 1:100. Gel Mobility Shift Assay. ELISA. Positive control: Recombinant Human HSF-1 Protein.
PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.