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Anti-HSF1 Antibody (clone 10H8) IHC-plus™ LS-B8141

Note: This antibody replaces LS-C150373
Catalog Size Price
LS-B8141-25 25 µg (1 mg/ml) Unavailable

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177 HSF1 Antibodies

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Anti-HSF1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2682 concentration 5 ug/ml.
Anti-HSF1 Antibody (clone J7F9) IHC-plus™ LS-B2951
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Anti-HSF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2951 concentration 15 ug/ml.
Anti-HSF1 Antibody (phospho-Ser303) LS-C117376
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IHC of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is treated with the synthesized peptide.
Anti-HSF1 Antibody (phospho-Ser121) LS-C381376
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Anti-HSF1 Antibody (aa15-288, Biotin) LS-C684501
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Western blot of recombinant HSF1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

100% Guaranteed
Rat Monoclonal [clone 10H8] (IgG1)
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Rabbit
IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation, ELISA, Gel shift (applications tested for the base form of this product only)
Unconjugated

Details

HSF1
Rat
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Rabbit (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [10H8]
Unconjugated. Also available conjugated with Atto 390, Atto 488, Atto 594, Atto 565, Atto 633, Atto 680, Atto 700, AP, Atto 655, Biotin, FITC, APC, PerCP, PE, Atto 594, RPE, SA.
Protein G purified from tissue culture supernatant
Unmodified

Applications

  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Western blot
  • Immunoprecipitation
  • ELISA
  • Gel shift
  • (applications tested for the base form of this product only)

Specificity and Use

HSF1 antibody was raised against purified recombinant HSF1
Detects an ~85kDa protein inunstressed cell lysates, and an~95kDa protein in heat shockedcell lysates, corresponding tothe molecular mass of inactiveand active forms of HSF1 onSDS-PAGE immunoblots.

Packaging

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.

About HSF1

Q00613 NM_005526 NP_005517.1

HSF1 Antibody, HSTF 1 Antibody, Heat shock factor protein 1 Antibody, HSF 1 Antibody, HSTF1 Antibody

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Immunohistochemistry

Anti-HSF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8141 dilution 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HSF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8141 dilution 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HSF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8141 dilution 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HSF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8141 dilution 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

HSF1 granules present in heat-shocked mitotic cells (green) detected by IF in mitotic heat shocked HeLa cells.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HSF1 granules present in heat-shocked mitotic cells (green) detected by IF in mitotic heat shocked HeLa cells. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/22/2018

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