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Anti-HSF1 Antibody (clone 10H8) IHC-plus™ LS-B8141

Note: This antibody replaces LS-C150373

Ordering

Wt. Vol. Conc. Price
25 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HSF1 Antibodies

Anti-HSF1 Antibody (clone J7F9) IHC-plus™ LS-B2951
Mouse Monoclonal [clone J7F9] (IgG1,k) to Human HSF1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone 10H8] (IgG1)
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Rabbit
IHC - Paraffin, ICC, Western blot, Immunoprecipitation, ELISA, Gel shift (applications tested for the base form of this product only)
Unconjugated

Details

HSF1
Rat
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Hamster, Rabbit (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [10H8]
Unconjugated. Also available conjugated with Atto 390, Atto 488, Atto 594, Atto 565, Atto 633, Atto 680, Atto 700, AP, Atto 655, Biotin, FITC, APC, PerCP, PE, Atto 594, RPE, SA.
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Western blot
  • Immunoprecipitation
  • ELISA
  • Gel shift
  • (applications tested for the base form of this product only)

Specificity and Use

HSF1 antibody was raised against purified recombinant HSF1
Detects an ~85kDa protein inunstressed cell lysates, and an~95kDa protein in heat shockedcell lysates, corresponding tothe molecular mass of inactiveand active forms of HSF1 onSDS-PAGE immunoblots.

Packaging

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About HSF1

Q00613 NM_005526 NP_005517.1

HSF1 Antibody, HSTF 1 Antibody, Heat shock factor protein 1 Antibody, HSF 1 Antibody, HSTF1 Antibody

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Immunohistochemistry

Immunohistochemistry
Anti-HSF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8141 dilution 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-HSF1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8141 dilution 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunofluorescence

Immunofluorescence
HSF1 granules present in heat-shocked mitotic cells (green) detected by IF in mitotic heat shocked HeLa cells. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/10/2016

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