LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
HSF1 Antibody (C‑Terminus) IHC‑plus™ LS‑B1539 Best Seller
Note: This antibody replaces LS-C30218
HSF1 antibody LS-B1539 is an unconjugated rabbit polyclonal antibody to HSF1 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B1539-50
50 µg
Unavailable

Popular HSF1 Products

Anti-HSF1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2682 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Anti-HSF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2951 concentration 15 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
IHC of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is treated with the synthesized peptide.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA

Product Description

HSF1 antibody LS-B1539 is an unconjugated rabbit polyclonal antibody to HSF1 from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About HSF1
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Q00613 NM_005526 NP_005517.1

HSF1 Antibody, HSTF 1 Antibody, Heat shock factor protein 1 Antibody, HSF 1 Antibody, HSTF1 Antibody


Specifications

Target
Human HSF1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.06 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HSF1 antibody was raised against synthetic peptide from C-Terminus of human HSF1 (Q00613, NP_005517). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Hamster, Zebu, Dog, Bovine, Horse, Pig (100%); Marmoset, Panda, Rabbit, Platypus (92%); Mouse, Rat, Guinea pig (85%).
Blocking Peptide
LS-E10826 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B1539. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human HSF1
Usage
Immunohistochemistry: LS-B1539 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1539 was determined to be 5 ug/ml.
Presentation
Lyophilized.
Reconstitution
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

Anti-HSF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1539 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HSF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1539 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HSF1 antibody LS-B1539 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HSF1 antibody LS-B1539 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HSF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1539 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HSF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1539 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HSF1 antibody LS-B1539 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HSF1 antibody LS-B1539 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HSF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1539 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HSF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1539 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HSF1 antibody LS-B1539 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HSF1 antibody LS-B1539 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HSF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1539 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HSF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1539 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HSF1 antibody LS-B1539 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HSF1 antibody LS-B1539 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number