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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSF1 Antibody (aa1‑503) LS‑C7365
HSF1 antibody LS-C7365 is an unconjugated rat monoclonal antibody to HSF1 from human, mouse and rat. Validated for IF, IP and WB.
Antibody
Catalog
Size
Price
LS-C7365-250
250 µl (0.1 mg/ml)
$425
LS-C7365-500
500 µl
$635

Popular HSF1 Products

Anti-HSF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1539 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot
Anti-HSF1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2682 concentration 5 ug/ml.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is blocked with the phospho peptide.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA

Product Description

HSF1 antibody LS-C7365 is an unconjugated rat monoclonal antibody to HSF1 from human, mouse and rat. Validated for IF, IP and WB.
About HSF1
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Q00613 NM_005526 NP_005517.1

HSF1 Antibody, HSTF 1 Antibody, Heat shock factor protein 1 Antibody, HSF 1 Antibody, HSTF1 Antibody


Specifications

Target
Mouse HSF1
Host
Rat
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • Immunoprecipitation
Immunogen
HSF1 antibody was raised against recombinant protein corresponding to aa1-503 of mouse HSF1. Epitope: aa425-439 of mouse HSF1. Cellular Localization: Nuclear.
Epitope
aa1-503
Specificity
Recognizes Heat Shock Factor 1 (HSF1) at 70-85kD, depending on the source and state of cells. Species cross-reactivity: Human, mouse and rat.
Usage
Suitable for use in Immunofluorescence, Immunoprecipitation and Western Blot. Immunoprecipitation (Native only): 2 ug/mg protein lysate using Protein G. Western Blot: 1-2 ug/ml. Positive control: LS174T or MAD109 cells.
Presentation
PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, sterile glycerol to 80%.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 7/20/2018

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