HSF1 antibody LS-C7365 is an unconjugated rat monoclonal antibody to HSF1 from human, mouse and rat. Validated for IF, IP and WB.
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
- Western blot (1 - 2 µg/ml)
HSF1 antibody was raised against recombinant protein corresponding to aa1-503 of mouse HSF1. Epitope: aa425-439 of mouse HSF1. Cellular Localization: Nuclear.
Recognizes Heat Shock Factor 1 (HSF1) at 70-85kD, depending on the source and state of cells. Species cross-reactivity: Human, mouse and rat.
Suitable for use in Immunofluorescence, Immunoprecipitation and Western Blot. Immunoprecipitation (Native only): 2 ug/mg protein lysate using Protein G. Western Blot: 1-2 ug/ml. Positive control: LS174T or MAD109 cells.
PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, sterile glycerol to 80%.
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.