HSF1 antibody LS-C7364 is an unconjugated rat monoclonal antibody to HSF1 from human, mouse, rat and other species. Validated for IF, IP and WB.
Mouse, Human, Monkey, Rat, Guinea pig
(tested or 100% immunogen sequence identity)
- Western blot (1:200 - 1:400)
HSF1 antibody was raised against full length recombinant mouse HSF1 protein (aa 1-503).
Recognizes a protein of 70-85kD (depending upon the source and state of cells), identified as Heat Shock Factor 1 (HSF1). Species cross-reactivity: Human, Rat, Mouse, Guinea Pig and Monkey.
Immunofluorescence (predominantly nuclear). Immunoprecipitation: 10 ul/mg cell lysate Western Blot: 1:200-1:400 (for 2hrs. At Room Temperature). Positive control: NIH-3T3, K562, HL-60, HEP-2, or HeLa cells.
PBS, pH 7.4, 0.1% Sodium Azide, 0.2% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.