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Anti-HSF1 Antibody (aa1-503, Azide-free) LS-C7367

Ordering

Wt. Vol. Conc. Price
100 µg - - $545
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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Rat Monoclonal (IgG1) to Mouse HSF1
Mouse, Human, Rat
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Gel shift
Unconjugated, Azide-free

Details

Mouse HSF1
Rat
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Ammonium sulfate precipitation
Azide-free

Applications

IHC - Paraffin, Immunofluorescence, Western blot (1 - 2 µg/ml), Immunoprecipitation, Gel shift

Specificity and Use

HSF1 antibody was raised against recombinant protein corresponding to aa1-503 of mouse HSF1. Epitope: aa 288-439 of mouse HSF1.
aa1-503
Recognizes mouse Heat Shock Factor 1 (HSF1) at 70-85kD, depending on the source and state of cells. Species cross-reactivity: Human, rat.
Suitable for use in Gel Supershift, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry. Gel Supershift: Antibody should be used at high concentration (1 mg/ml). Immunoprecipitation: 2 ug antibody/mg protein lysate using Protein G (Native only). Western Blot: 1-2 ug/ml for 2hrs at RT. Immunohistochemistry: 29-4 ug/ml. Formalin/paraffin for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: LS174T cells. Breast carcinoma.

Packaging

PBS, pH 7.4.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About HSF1

Q00613 NM_005526 NP_005517.1

HSF1 Antibody, HSTF 1 Antibody, Heat shock factor protein 1 Antibody, HSF 1 Antibody, HSTF1 Antibody

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Requested From: United States
Date Requested: 9/29/2016

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