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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HSF1 Antibody (aa1‑503, Azide‑free) LS‑C7367
HSF1 antibody LS-C7367 is an unconjugated rat monoclonal antibody to HSF1 from human, mouse and rat. Validated for GS, IF, IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C7367-100
100 µg
$605

Popular HSF1 Products

Anti-HSF1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2951 concentration 15 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-HSF1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2682 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSF1 (Phospho-Ser303) Antibody. The picture on the right is blocked with the phospho peptide.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-HSF1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1539 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA

Product Description

HSF1 antibody LS-C7367 is an unconjugated rat monoclonal antibody to HSF1 from human, mouse and rat. Validated for GS, IF, IHC, IP and WB.
About HSF1
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Q00613 NM_005526 NP_005517.1

HSF1 Antibody, HSTF 1 Antibody, Heat shock factor protein 1 Antibody, HSF 1 Antibody, HSTF1 Antibody


Specifications

Target
Mouse HSF1
Host
Rat
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Ammonium sulfate precipitation
Modifications
Azide-free
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • Immunoprecipitation
  • Gel shift
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HSF1 antibody was raised against recombinant protein corresponding to aa1-503 of mouse HSF1. Epitope: aa 288-439 of mouse HSF1.
Epitope
aa1-503
Specificity
Recognizes mouse Heat Shock Factor 1 (HSF1) at 70-85kD, depending on the source and state of cells. Species cross-reactivity: Human, rat.
Usage
Suitable for use in Gel Supershift, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry. Gel Supershift: Antibody should be used at high concentration (1 mg/ml). Immunoprecipitation: 2 ug antibody/mg protein lysate using Protein G (Native only). Western Blot: 1-2 ug/ml for 2hrs at RT. Immunohistochemistry: 29-4 ug/ml. Formalin/paraffin for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: LS174T cells. Breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/22/2018

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