Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Gel shift
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
IHC - Paraffin, Immunofluorescence, Western blot (1 - 2 µg/ml), Immunoprecipitation, Gel shift
Specificity and Use
HSF1 antibody was raised against recombinant protein corresponding to aa1-503 of mouse HSF1. Epitope: aa 288-439 of mouse HSF1.
Recognizes mouse Heat Shock Factor 1 (HSF1) at 70-85kD, depending on the source and state of cells. Species cross-reactivity: Human, rat.
Suitable for use in Gel Supershift, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry. Gel Supershift: Antibody should be used at high concentration (1 mg/ml). Immunoprecipitation: 2 ug antibody/mg protein lysate using Protein G (Native only). Western Blot: 1-2 ug/ml for 2hrs at RT. Immunohistochemistry: 29-4 ug/ml. Formalin/paraffin for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: LS174T cells. Breast carcinoma.
PBS, pH 7.4.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C