Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Ammonium sulfate precipitation
- IHC - Paraffin
- Western blot (1 - 2 µg/ml)
- Gel shift
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
HSF1 antibody was raised against recombinant protein corresponding to aa1-503 of mouse HSF1. Epitope: aa 288-439 of mouse HSF1.
Recognizes mouse Heat Shock Factor 1 (HSF1) at 70-85kD, depending on the source and state of cells. Species cross-reactivity: Human, rat.
Suitable for use in Gel Supershift, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry. Gel Supershift: Antibody should be used at high concentration (1 mg/ml). Immunoprecipitation: 2 ug antibody/mg protein lysate using Protein G (Native only). Western Blot: 1-2 ug/ml for 2hrs at RT. Immunohistochemistry: 29-4 ug/ml. Formalin/paraffin for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: LS174T cells. Breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.