Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
HSF1 antibody was raised against recombinant protein corresponding to aa1-503 of mouse HSF1. Epitope: aa 288-439 of mouse HSF1.
Recognizes mouse Heat Shock Factor 1 (HSF1) at 70-85kD, depending on the source and state of cells. Species cross-reactivity: Human, rat.
Suitable for use in Gel Supershift, Immunofluorescence, Immunoprecipitation, Western Blot, Immunohistochemistry. Gel Supershift: Antibody should be used at high concentration (1 mg/ml). Immunoprecipitation: 2 ug antibody/mg protein lysate using Protein G (Native only). Western Blot: 1-2 ug/ml for 2hrs at RT. Immunohistochemistry: 29-4 ug/ml. Formalin/paraffin for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: LS174T cells. Breast carcinoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
PBS, pH 7.4
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.