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Anti-HSD11B1 / HSD11B Antibody (aa64-77) LS-C154869

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular HSD11B1 / HSD11B Antibodies

Anti-HSD11B1 / HSD11B Antibody (aa77-92) IHC-plus™ LS-B1597
Rabbit Polyclonal (IgG) to Human HSD11B1 / HSD11B
Human, Monkey, Mouse, Rat, Hamster
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HSD11B1 / HSD11B Antibody (aa131-145) LS-C172267
Rabbit Polyclonal to Human HSD11B1 / HSD11B
Human, Mouse, Rat
IHC - Paraffin, IHC - Frozen, ICC, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Mouse HSD11B1 / HSD11B
Mouse, Rat, Hamster
Western blot, ELISA
Unconjugated

Details

Mouse HSD11B1 / HSD11B
Goat
Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.3 - 1 µg/ml)
  • ELISA (1:32000)

Specificity and Use

HSD11B1 / HSD11B antibody was raised against synthetic peptide C-TARSEEGLQKVVSR from an internal region of mouse HSD11B1 (NP_032314.2). Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%); Galago (93%); Sheep, Bovine, Horse (86%).
LS-E27741 - Lyophilized - 100 µg - $145.00
aa64-77
Mouse HSD11B1.
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 32kD band observed in Mouse Lung lysates (calculated MW of 32.4kD according to NP_032314.2). Recommended concentration: 0.3-1 ug/ml. An additional band of unknown identity was also consistently observed at 45kD. This band was successfully blocked by incubation with the immunizing peptide. However, it remained visible in a KO background.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About HSD11B1 / HSD11B

P28845 NM_005525 NP_005516.1

HSD11B1 Antibody, 11-beta-HSD1 Antibody, 11-DH Antibody, CORTRD2 Antibody, HSD11B Antibody, HSD11L Antibody, SDR26C1 Antibody, HDL Antibody, HSD11 Antibody

HSD11B1 / HSD11B is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children.

Western blot

Western blot
HSD11B1 antibody (0.3 ug/ml) staining of Mouse Lung lysate (60 ug protein in RIPA buffer). First lane shows wild type and second lane shows knockout background. Primary incubation was overnight. Detected by fluorescence with Li-cor). Data obtained from Prof. K. Chapman and Zhenguang Zhang, Centre for Cardiovascular Sciences, Queens Medical Research Institute, Edinburgh, UK

Western blot

Western blot
HSD11B1 antibody (0.3 ug/ml) staining of Mouse Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/2/2016

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