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HSD-1 / SPAG8

sperm associated antigen 8

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. This protein interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator)-ACT-mediated gene transcription during spermatogenesis. This protein may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

Gene Name: sperm associated antigen 8
Synonyms: SPAG8, Sperm membrane protein BS-84, Sperm-associated antigen 8, SMP-1, Sperm associated antigen 8, SMP1, SPAG3, BS-84, HSD-1, HSMP-1, Sperm membrane protein 1
Target Sequences: BC019247 AAH19247.1 Q99932

☰ Filters
Antibodies (22)
Proteins (4)
Over-Expression Lysate (2)
Primary (22)
Recombinant (2)
HSD-1 / SPAG8 (26)
Human (21)
Mouse (4)
Rat (4)
Gibbon (3)
Monkey (3)
IHC (2)
WB (18)
ELISA (9)
IF (1)
IP (2)
rabbit (22)
IgG (20)
polyclonal pc (22)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (4)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (1)
Unconjugated (8)
aa383-411 (7)
aa143-192 (4)
aa396-445 (4)
No (26)
GST (1)
GST, N-terminus (1)
Myc-DDK (Flag) (2)
Human (3)
293T Cells (1)
HEK 293 Cells (1)
Wheat Germ Extract (2)
Purified (1)
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IP, WB
Unconjugated
100 µg/$341
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HSD-1 / SPAG8 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat
IHC, IP, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
HSD-1 / SPAG8 Antibody - Immunofluorescence staining of PC3 cells at a dilution of 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 °C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L) .
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HSD-1 / SPAG8 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF
Unconjugated
50 µg/$294; 100 µg/$360
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (FITC) Antibody
Human
FITC Conjugated
50 µg/$294; 100 µg/$360
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa383-411) Antibody
Human
ELISA, WB
Unconjugated
200 µl/$734
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa383-411) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
200 µl/$952
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa383-411) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
200 µl/$952
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa383-411) (Azide-free) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated, Azide-free
200 µl/$952
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa383-411) (APC) Antibody
Human
ELISA, WB
APC Conjugated
200 µl/$952
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa383-411) (PE) Antibody
Human
ELISA, WB
PE Conjugated
200 µl/$952
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$357
HSD-1 / SPAG8 Antibody - SPAG8 antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa143-192) Antibody
Human
WB
Unconjugated
100 µl/$424
HSD-1 / SPAG8 Antibody - SPAG8 antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa143-192) (HRP) Antibody
Human
WB
HRP Conjugated
100 µl/$469
HSD-1 / SPAG8 Antibody - SPAG8 antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa143-192) (Biotin) Antibody
Human
WB
Biotin Conjugated
100 µl/$469
HSD-1 / SPAG8 Antibody - SPAG8 antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa143-192) (FITC) Antibody
Human
WB
FITC Conjugated
100 µl/$469
HSD-1 / SPAG8 Antibody - SPAG8 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa396-445) Antibody
Human
WB
Unconjugated
100 µl/$424
HSD-1 / SPAG8 Antibody - SPAG8 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa396-445) (HRP) Antibody
Mouse, Rat, Gibbon, Human, Monkey
WB
HRP Conjugated
100 µl/$469
HSD-1 / SPAG8 Antibody - SPAG8 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa396-445) (Biotin) Antibody
Mouse, Rat, Gibbon, Human, Monkey
WB
Biotin Conjugated
100 µl/$469
HSD-1 / SPAG8 Antibody - SPAG8 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa396-445) (FITC) Antibody
Mouse, Rat, Gibbon, Human, Monkey
WB
FITC Conjugated
100 µl/$469
HSD-1 / SPAG8 Antibody - SPAG8 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The SPAG8 antibody detected the SPAG8 protein (arrow).
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HSD-1 / SPAG8 Rabbit anti-Human Polyclonal (aa383-411) Antibody
Human
WB
Unconjugated
400 µl/$393
HSD-1 / SPAG8 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
53.3 kDa
20 µg/$150
HSD-1 / SPAG8 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
53.3 kDa
100 µg/$494
HSD-1 / SPAG8 Protein - 12.5% SDS-PAGE of human SPAG8 stained with Coomassie Blue
Select
Wheat Germ Extract
GST
80.85 kDa
10 µg/$479; 25 µg/$670
Viewing 1-25 of 26 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).