Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factor involved in the HS response.
HeLa cells staining with HSBP1 antibody (red), and counterstained with chicken polyclonal antibody to Vimentin (green) and DNA (blue). The HSBP1 antibody antibody reveals strong cytoplasmic staining and penetrates into the actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.
Western blots of HeLa cell crude extracts. Lane 16 was probed with HSBP1 antibody, while lanes 14 and 15 were probed with two other monoclonals to HSP27 we generated at the same time. Note the strong clean bands at 27 kDa. Lane 17 was probed with MCA-4C4, our new mouse monoclonal antibody to Lamia A/C, which binds two bands running at 74 kDa and 65 kDa. Lane 13 was probed with MCA-5J11, our monoclonal antibody to all six actin isotypes. Molecular weights of each protein are as indicated, and dots indicate the presence of major HeLa proteins.
Requested From: United States
Date Requested: 10/26/2016