Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Rat, Bovine, Dog, Pig, Rabbit, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000), ICC (1:100 - 1:1000), Western blot (1:500 - 1:3000)
Specificity and Use
HPRT1 / HPRT antibody was raised against recombinant fragment corresponding to a region within amino acids 23 and 218 of HPRT (SwissProt P00492). Percent identity by BLAST analysis: Human (100%); Rabbit (98%); Pig (97%); Dog, Rat (96%); Chicken, Bovine (94%); Zebrafish (93%); Xenopus (92%).
Human HPRT1 / HPRT
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.