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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HPD Antibody (aa92‑121) LS‑C164997
HPD antibody LS-C164997 is an unconjugated rabbit polyclonal antibody to HPD from human and mouse. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C164997-400
400 µl
Unavailable

Popular HPD Products

HPD antibody LS-C107620 Western blot of Fetal Spleen lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish
Applications: Western blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Immunohistochemistry of paraffin-embedded mouse kidney tissue.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemical analysis of HPPDase staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

HPD antibody LS-C164997 is an unconjugated rabbit polyclonal antibody to HPD from human and mouse. Validated for Flow, IHC and WB.
About HPD
HPD is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene. P32754 NM_002150 NP_002141.1

HPD Antibody, 4HPPD Antibody, 4-HPPD Antibody, GLOD3 Antibody, HPPDASE Antibody, PPD Antibody, Glyoxalase domain containing 3 Antibody


Specifications

Target
Human HPD
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9662 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C164997. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa92-121
Specificity
This HPD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-121 amino acids from the N-terminal region of human HPD.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of HPD Antibody in HepG2 cell line lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.
Western blot of HPD Antibody in HepG2 cell line lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.

Western blot

Western blot of HPD Antibody in mouse liver tissue lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.
Western blot of HPD Antibody in mouse liver tissue lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of HPD Antibody in HepG2 cell line lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.
Western blot of HPD Antibody in HepG2 cell line lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.

Western blot

Western blot of HPD Antibody in mouse liver tissue lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.
Western blot of HPD Antibody in mouse liver tissue lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of HPD Antibody in HepG2 cell line lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.
Western blot of HPD Antibody in HepG2 cell line lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.

Western blot

Western blot of HPD Antibody in mouse liver tissue lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.
Western blot of HPD Antibody in mouse liver tissue lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of HPD Antibody in HepG2 cell line lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.
Western blot of HPD Antibody in HepG2 cell line lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.

Western blot

Western blot of HPD Antibody in mouse liver tissue lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.
Western blot of HPD Antibody in mouse liver tissue lysates (35 ug/lane). HPD (arrow) was detected using the purified antibody.


Requested From: 
Date Requested: 6/24/2018

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