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Anti-HP / Haptoglobin Antibody IHC-plus™ LS-B32

Note: This antibody replaces LS-C18817

Ordering

Wt. Vol. Conc. Price
50 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HP / Haptoglobin Antibodies

Anti-HP / Haptoglobin Antibody IHC-plus™ LS-B2266
Rabbit Polyclonal (IgG) to Human HP / Haptoglobin
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HP / Haptoglobin Antibody (clone 2F4) IHC-plus™ LS-B2863
Mouse Monoclonal [clone 2F4] (IgG1,k) to Human HP / Haptoglobin
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HP / Haptoglobin Antibody IHC-plus™ LS-B13232
Goat Polyclonal to Human HP / Haptoglobin
Human
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Immunodiffusion
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal to Human HP / Haptoglobin
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human HP / Haptoglobin
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (0.31 µg/ml)
  • Immunofluorescence
  • Western blot (1:2000 - 1:10000)
  • ELISA

Specificity and Use

Human Haptoglobin.
Immunohistochemistry: LS-B32 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B32 was determined to be 0.31 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

Publications (2)

1
Combination of haptoglobin and osteopontin could predict colorectal cancer hepatic metastasis. Sun L, Pan J, Peng L, Fang L, Zhao X, Sun L, Yang Z, Ran Y. Annals of surgical oncology. 2012 Jul;19:2411-9. [Full Text Article] [PubMed:22219064]
2
The extracellular chaperone haptoglobin prevents serum fatty acid-promoted amyloid fibril formation of beta 2-microglobulin, resistance to lysosomal degradation, and cytotoxicity. Sultan A, Raman B, Rao ChM, Tangirala R. The Journal of biological chemistry. 2013 Nov;288:32326-42. [Full Text Article] [PubMed:24078632] [PMC:PMC3820869]

About HP / Haptoglobin

P00738 NM_005143 NP_005134.1

HP Antibody, Binding peptide Antibody, Haptoglobin alpha(1S)-beta Antibody, Haptoglobin, beta polypeptide Antibody, Haptoglobin Antibody, Haptoglobin alpha(2FS)-beta Antibody, Haptoglobin, alpha polypeptide Antibody, HP2ALPHA2 Antibody, BP Antibody, Zonulin Antibody, HPA1S Antibody

HP / Haptoglobin is a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia.

Immunohistochemistry

Immunohistochemistry
Anti-Haptoglobin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B32 concentration 31 ug/ml.
 

Requested From: United States
Date Requested: 12/10/2016

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