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Anti-HNRNP-E2 / PCBP2 Antibody (clone 5F12) IHC-plus™ LS-B4392

Note: This antibody replaces LS-C105009

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HNRNP-E2 / PCBP2 Antibodies

Anti-HNRNP-E2 / PCBP2 Antibody (aa31-80) LS-C80352
Rabbit Polyclonal (IgG) to Human HNRNP-E2 / PCBP2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Western blot
Unconjugated
Western blot Image
Anti-HNRNP-E2 / PCBP2 Antibody (clone 5F12) IHC-plus™ LS-B4392
Mouse Monoclonal [clone 5F12] (IgG2a,k) to Human HNRNP-E2 / PCBP2
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA, RNA interference
Unconjugated
Immunohistochemistry Image
Anti-HNRNP-E2 / PCBP2 Antibody IHC-plus™ LS-B13379
Rabbit Polyclonal (IgG) to Human HNRNP-E2 / PCBP2
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5F12] (IgG2a,k) to Human HNRNP-E2 / PCBP2
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA, RNA interference
Unconjugated

Details

Human HNRNP-E2 / PCBP2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [5F12]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
  • RNA interference

Specificity and Use

HNRNP-E2 / PCBP2 antibody was raised against pCBP2 (AAH01155, 1 a.a. ~ 363 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control K-562. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated).

Packaging

PBS, pH 7.2. Sourced in Ascites.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About HNRNP-E2 / PCBP2

Q15366 NM_005016 NP_005007.2

PCBP2 Antibody, Alpha-CP2 Antibody, HnRNP E2 Antibody, HNRPE2 Antibody, Poly(rC) binding protein 2 Antibody, Poly(rC)-binding protein 2 Antibody, HnRNP-E2 Antibody

HNRNP-E2 / PCBP2 appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure.

Immunohistochemistry

Immunohistochemistry
Anti-PCBP2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4392 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-PCBP2 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4392 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to PCBP2 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

Western blot
Western blot of PCBP2 expression in transfected 293T cell line by PCBP2 monoclonal antibody clone 5F12.

Western blot

Western blot
PCBP2 monoclonal antibody clone 5F12 Western blot of PCBP2 expression in K-562.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PCBP2 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of PCBP2 over-expressed 293 cell line, cotransfected with PCBP2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PCBP2 monoclonal antibody clone 5F12. GAPDH ( 36.1 kD ) used as specificity.
 

Requested From: United States
Date Requested: 12/6/2016

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