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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HNF4A / HNF4 Antibody (Ser142) LS‑C97257
HNF4 antibody LS-C97257 is an unconjugated rabbit polyclonal antibody to human HNF4 (HNF4A). Validated for WB.
Catalog
Size
Price
LS-C97257-400
400 µl
Unavailable

Popular HNF4A / HNF4 Products

Anti-HNF4A / HNF4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B969 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Anti-HNF4A / HNF4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3005 concentration 2 ug/ml.
Species: Human, Monkey, Bat, Dog, Hamster, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
HNF4A Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Anti-HNF4A / HNF4 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4568 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Confocal immunofluorescence of HNF4A Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Species: Human
Applications: Immunofluorescence, Western blot

Product Description

HNF4 antibody LS-C97257 is an unconjugated rabbit polyclonal antibody to human HNF4 (HNF4A). Validated for WB.
About HNF4A / HNF4
Hepatocyte nuclear factor 4 alpha (HNF4 alpha), a NR2 Hepatocyte NF4-Like receptor, is a liver-enriched transcription factor that is involved in the expression of liver-specific genes. HNF4 alpha controls the expression of transcription factor HNF1, which is important in the expression of hepatic genes, including those involved in glucose, cholesterol, and fatty acid metabolism. P41235 NM_000457 NP_000448.3

HNF4A Antibody, Hepatic nuclear factor 4 alpha Antibody, HNF4 Antibody, HNF4a8 Antibody, NR2A21 Antibody, TCF Antibody, TCF-14 Antibody, TCF14 Antibody, MODY1 Antibody, Transcription factor 14 Antibody, Transcription factor HNF-4 Antibody, HNF-4-alpha Antibody, HNF4 alpha Antibody, HNF4a7 Antibody, HNF4a9 Antibody, HNF4alpha Antibody, HNF4alpha10/11/12 Antibody, MODY Antibody, NR2A1 Antibody


Specifications

Target
Human HNF4A / HNF4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
Blocking Peptide
LS-E7089 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C97257. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Ser142
Specificity
This HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-146 amino acids from human HNF4A.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of anti-HNF4A Antibody (S142) in NCI-H460 cell line lysates (35 ug/lane). HNF4A (arrow) was detected using the purified antibody.
Western blot of anti-HNF4A Antibody (S142) in NCI-H460 cell line lysates (35 ug/lane). HNF4A (arrow) was detected using the purified antibody.

Western blot

Western blot of anti-HNF4A Antibody (S142) in NCI-H460 cell line lysates (35 ug/lane). HNF4A (arrow) was detected using the purified antibody.
Western blot of anti-HNF4A Antibody (S142) in NCI-H460 cell line lysates (35 ug/lane). HNF4A (arrow) was detected using the purified antibody.

Western blot

Western blot of anti-HNF4A Antibody (S142) in NCI-H460 cell line lysates (35 ug/lane). HNF4A (arrow) was detected using the purified antibody.
Western blot of anti-HNF4A Antibody (S142) in NCI-H460 cell line lysates (35 ug/lane). HNF4A (arrow) was detected using the purified antibody.

Western blot

Western blot of anti-HNF4A Antibody (S142) in NCI-H460 cell line lysates (35 ug/lane). HNF4A (arrow) was detected using the purified antibody.
Western blot of anti-HNF4A Antibody (S142) in NCI-H460 cell line lysates (35 ug/lane). HNF4A (arrow) was detected using the purified antibody.


Requested From: 
Date Requested: 6/24/2018

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