Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Hepatocyte nuclear factor 4 alpha (HNF4 alpha), a NR2 Hepatocyte NF4-Like receptor, is a liver-enriched transcription factor that is involved in the expression of liver-specific genes. HNF4 alpha controls the expression of transcription factor HNF1, which is important in the expression of hepatic genes, including those involved in glucose, cholesterol, and fatty acid metabolism.
Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody (M04), clone 4E2.Lane 1: HNF4A transfected lysate(51.6 KDa).Lane 2: Non-transfected lysate.
HNF4A monoclonal antibody (M04), clone 4E2 Western Blot analysis of HNF4A expression in HepG2.
HNF4A monoclonal antibody (M04), clone 4E2. Western Blot analysis of HNF4A expression in Jurkat.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged HNF4A is approximately 1 ng/ml as a capture antibody.
Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HNF4A monoclonal antibody (M04), clone 4E2 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Requested From: United States
Date Requested: 1/19/2017