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Anti-HMOX1 / HO-1 Antibody (clone 5F12-B10) IHC-plus™ LS-B3407

Note: This antibody replaces LS-C103500

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HMOX1 / HO-1 Antibodies

Anti-HMOX1 / HO-1 Antibody IHC-plus™ LS-B3421
Rabbit Polyclonal to Human HMOX1 / HO-1
Human, Monkey, Mouse, Rat, Dog, Hamster, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5F12-B10] (IgG1) to Human HMOX1 / HO-1
Human, Mouse, Rat, Bovine, Dog
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human HMOX1 / HO-1
Mouse
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [5F12-B10]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:250)
  • Flow Cytometry

Specificity and Use

HMOX1 / HO-1 antibody was raised against synthetic peptide derived from sequence near the amino-terminus of human HO-1 (Hsp32).
Immunohistochemistry: LS-B3407 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3407 was determined to be 10 ug/ml.

Packaging

PBS, 0.09% azide, 50% glycerol
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About HMOX1 / HO-1

P09601 NM_002133 NP_002124.1

HMOX1 Antibody, BK286B10 Antibody, HO-1 Antibody, HSP32 Antibody, Heat shock protein, 32-kD Antibody, Heme oxygenase (decycling) 1 Antibody, Heme oxygenase 1 Antibody, HO1 Antibody

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

Immunohistochemistry

Immunohistochemistry
Anti-HMOX1 / Heme Oxygenase 1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3407 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-HMOX1 / Heme Oxygenase 1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3407 concentration 10 ug/ml.

Requested From: United States
Date Requested: 12/8/2016

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