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Anti-HMGN1 / HMG14 Antibody (phospho-Ser20/Ser24) IHC-plus™ LS-B54

Note: This antibody replaces LS-C18917


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular HMGN1 / HMG14 Antibodies

Anti-HMGN1 / HMG14 Antibody (phospho-Ser20/Ser24) IHC-plus™ LS-B54
Rabbit Polyclonal to Human HMGN1 / HMG14
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-HMGN1 / HMG14 Antibody (C-Terminus) IHC-plus™ LS-B10268
Rabbit Polyclonal to Human HMGN1 / HMG14
Human, Monkey, Bovine, Horse, Pig
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human HMGN1 / HMG14
IHC - Paraffin, Western blot, ELISA


Human HMGN1 / HMG14
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (20 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:25000)

Specificity and Use

HMGN1 / HMG14 antibody was raised against synthetic peptide from human HMGN1.
Amino acids 19-28 of human HMGN protein containing pSer20 and pSer24
Immunohistochemistry: LS-B54 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B54 was determined to be 20 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HMGN1 / HMG14

P05114 NM_004965 NP_004956.5

HMGN1 Antibody, HMG14 Antibody

HMGN1 binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. HMGN1 has been shown to enhance transcription on chromatin templates but not on DNA templates suggesting that the association of HMGN1 with nucleosomes is part of the cellular process involved in the generation of transcriptionally active chromatin. During mitosis HMGN1 is phosphorylated in its chromatin binding domain.


Anti-HMGN1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B54 concentration 20 ug/ml.

Western blot

Western blot
Anti-HMNG Antibody - Western Blot. Western blot of Affinity Purified anti-HMNG antibody shows detection of phosphorylated HMGN1 and HMGN2. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lanes contain: 1 - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1000 for this experiment. The blot was procebetaed using a 5 sec exposure time. Personal Communication, Yuri Postnikov, NIH, CCR, Bethesda, MD.

Requested From: United States
Date Requested: 10/21/2016

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