LSBio The Immunohistochemistry Antibody Company
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All ELISA Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-HMGN1 / HMG14 Antibody (phospho-Ser20/Ser24) IHC-plus™ LS-B54

Note: This antibody replaces LS-C18917


Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

Related Products

41 HMGN1 / HMG14 Antibodies

Most Popular HMGN1 / HMG14 Antibodies

Anti-HMGN1 / HMG14 Antibody (phospho-Ser20/Ser24) IHC-plus™ LS-B54
Rabbit Polyclonal to Human HMGN1 / HMG14
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-HMGN1 / HMG14 Antibody (aa36-85) IHC-plus™ LS-B3496
Rabbit Polyclonal (IgG) to Human HMGN1 / HMG14
Human, Monkey, Mouse, Rat, Bovine, Horse
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-HMGN1 / HMG14 Antibody (C-Terminus) IHC-plus™ LS-B10268
Rabbit Polyclonal to Human HMGN1 / HMG14
Human, Monkey, Bovine, Horse, Pig
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-HMGN1 / HMG14 Antibody LS-C408922
Rabbit Polyclonal (IgG) to Human HMGN1 / HMG14
Human, Mouse
IHC, Western blot
Anti-HMGN1 / HMG14 Antibody IHC-plus™ LS-B14060
Rabbit Polyclonal to Human HMGN1 / HMG14
IHC - Paraffin, Western blot
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human HMGN1 / HMG14
IHC - Paraffin, Western blot, ELISA


Human HMGN1 / HMG14
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (20 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:25000)

Specificity and Use

HMGN1 / HMG14 antibody was raised against synthetic peptide from human HMGN1.
Amino acids 19-28 of human HMGN protein containing pSer20 and pSer24
Immunohistochemistry: LS-B54 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B54 was determined to be 20 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HMGN1 / HMG14

P05114 NM_004965 NP_004956.5

HMGN1 Antibody, HMG14 Antibody

HMGN1 binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. HMGN1 has been shown to enhance transcription on chromatin templates but not on DNA templates suggesting that the association of HMGN1 with nucleosomes is part of the cellular process involved in the generation of transcriptionally active chromatin. During mitosis HMGN1 is phosphorylated in its chromatin binding domain.


Anti-HMGN1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B54 concentration 20 ug/ml.

Western blot

Western blot
Anti-HMNG Antibody - Western Blot. Western blot of Affinity Purified anti-HMNG antibody shows detection of phosphorylated HMGN1 and HMGN2. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lanes contain: 1 - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1000 for this experiment. The blot was procebetaed using a 5 sec exposure time. Personal Communication, Yuri Postnikov, NIH, CCR, Bethesda, MD.

Requested From: 
Date Requested: 4/24/2017

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2017 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number