Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
HMGA1 Antibody, High mobility group protein A1 Antibody, High mobility group protein R Antibody, HMG-R Antibody, Hmg-i Antibody, HMGIY Antibody, High mobility group AT-hook 1 Antibody, HMG-I(Y) Antibody, HMGA1A Antibody
HMGIY / HMGA1 is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins.
Fluorescent image of A549 cell stained with HMGA1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HMGA1 immunoreactivity is localized to Nucleus significantly.
HMGA1 Antibody western blot of K562 cell line lysates (35 ug/lane). The HMGA1 antibody detected the HMGA1 protein (arrow).
HMGA1 Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Requested From: United States
Date Requested: 2/26/2017