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Anti-HMGIY / HMGA1 Antibody (aa64-93) LS-C164930

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

Related Products

37 HMGIY / HMGA1 Antibodies

Most Popular HMGIY / HMGA1 Antibodies

Anti-HMGIY / HMGA1 Antibody (aa1-50) IHC-plus™ LS-B2037
Rabbit Polyclonal to Human HMGIY / HMGA1
Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HMGIY / HMGA1 Antibody (aa12-23) IHC-plus™ LS-B3008
Goat Polyclonal to Human HMGIY / HMGA1
Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HMGIY / HMGA1 Antibody (aa9-21) IHC-plus™ LS-B3972
Goat Polyclonal to Human HMGIY / HMGA1
Human, Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HMGIY / HMGA1 Antibody (aa64-93) LS-C164930
Rabbit Polyclonal to Human HMGIY / HMGA1
Human, Mouse
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunofluorescence Image
Anti-HMGIY / HMGA1 Antibody (aa1-53) IHC-plus™ LS-B11596
Rabbit Polyclonal (IgG) to Human HMGIY / HMGA1
Human, Mouse, Rat, Dog, Pig
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human HMGIY / HMGA1
Human, Mouse
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human HMGIY / HMGA1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E9942 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C164930. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa64-93
This HMGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the C-terminal region of human HMGA1.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About HMGIY / HMGA1

NM_002131 NP_002122.1

HMGA1 Antibody, High mobility group protein A1 Antibody, High mobility group protein R Antibody, HMG-R Antibody, Hmg-i Antibody, HMGIY Antibody, High mobility group AT-hook 1 Antibody, HMG-I(Y) Antibody, HMGA1A Antibody

HMGIY / HMGA1 is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins.

Immunofluorescence

Immunofluorescence
Fluorescent image of A549 cell stained with HMGA1 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). HMGA1 immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot
HMGA1 Antibody western blot of K562 cell line lysates (35 ug/lane). The HMGA1 antibody detected the HMGA1 protein (arrow).

Flow Cytometry

Flow Cytometry
HMGA1 Antibody flow cytometry of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 4/24/2017

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