Human HMGCS1 / HMG-CoA Synthase 1

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with Biotin, FITC, HRP.

Immunoaffinity purified

Unmodified

Synthetic peptide located between aa251-300 of human HMGCS1 (Q01581, NP_002121). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%); Marmoset, Bat, Guinea pig (92%); Opossum (85%).

aa251-300

Human HMGCS1

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1 µg/ml)

Immunohistochemistry: LS-B4016 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4016 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

PBS, 0.09% sodium azide, 2% sucrose

Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q01581 NCBI: NM_002130 NP_002121.4