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Anti-HMG20B / BRAF35 Antibody (aa201-250) LS-C110071

Catalog Size Price
LS-C110071-50 50 µg Unavailable

Most Popular HMG20B / BRAF35 Antibodies

Anti-HMG20B / BRAF35 Antibody (clone 1F6) LS-C133638
Mouse Monoclonal [clone 1F6] (IgG2a,k) to Human HMG20B / BRAF35
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
Immunofluorescence of monoclonal antibody to HMG20B on HeLa cell. [antibody concentration 10 ug/ml]
Anti-HMG20B / BRAF35 Antibody (Thr29) LS-C176838
Rabbit Polyclonal to Human HMG20B / BRAF35
Human
IHC, Immunofluorescence, Western blot
Unconjugated
Western blot of BRAF35 (T29) pAb in extracts from Jurkat cells.
Anti-HMG20B / BRAF35 Antibody (N-Terminus) LS-C356102
Rabbit Polyclonal to Human HMG20B / BRAF35
Human
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of BRAF35 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-HMG20B / BRAF35 Antibody (aa10-90) LS-C382305
Rabbit Polyclonal (IgG) to Human HMG20B / BRAF35
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot of BRAF35 antibody
Anti-HMG20B / BRAF35 Antibody LS-C663411
Rabbit Polyclonal to Mouse HMG20B / BRAF35
Mouse
IHC, ICC, Western blot, Immunoprecipitation
Unconjugated
Western Blot; Sample: Recombinant protein.

100% Guaranteed
Rabbit Polyclonal to Human HMG20B / BRAF35
Human, Mouse, Rat, Bovine, Guinea pig
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human HMG20B / BRAF35
Rabbit
Human, Mouse, Rat, Bovine, Guinea pig (tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

HMG20B / BRAF35 antibody was raised against synthetic peptide located between aa201-250 of human HMG20B (Q9P0W2, NP_006330). Percent identity by BLAST analysis: Human, Chimpanzee, Mouse, Bovine, Guinea pig (100%); Bat (84%).
LS-E12730 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110071. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa201-250
Human HMG20B / BRAF35
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HMG20B / BRAF35

Q9P0W2 NM_006339 NP_006330.2

HMG20B Antibody, BRAF35 Antibody, BRAF25 Antibody, BRCA2-associated factor 35 Antibody, High-mobility group 20B Antibody, HMGXB2 Antibody, High mobility group 20B Antibody, HMG box domain containing 2 Antibody, HMG box-containing protein 20B Antibody, HMGX2 Antibody, SMARCE1-related protein Antibody, PP7706 Antibody, Pp8857 Antibody, SMARCE1r Antibody, SOXL Antibody

Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.

Western blot

HMG20B / BRAF35 antibody LS-C110071 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMG20B / BRAF35 antibody LS-C110071 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/23/2018

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