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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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HMG2 / HMGB2 Antibody (aa72‑121) LS‑C112165
HMGB2 antibody LS-C112165 is an unconjugated rabbit polyclonal antibody to HMGB2 (HMG2) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C112165-50
50 µg
Unavailable

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Product Description

HMGB2 antibody LS-C112165 is an unconjugated rabbit polyclonal antibody to HMGB2 (HMG2) from human, mouse, rat and other species. Validated for WB.
About HMG2 / HMGB2
HMG2 / HMGB2 is a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. P26583 NM_002129 NP_002120.1

HMGB2 Antibody, HMG-2 Antibody, High mobility group box 2 Antibody, High-mobility group box 2 Antibody, HMG2 Antibody, High mobility group protein 2 Antibody, High mobility group protein B2 Antibody


Specifications

Target
Human HMG2 / HMGB2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
HMG2 / HMGB2 antibody was raised against synthetic peptide located between aa72-121 of human HMGB2 (P26583, NP_002120). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum (100%); Turkey, Chicken (92%); Guinea pig, Xenopus (86%).
Blocking Peptide
LS-E21283 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C112165. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa72-121
Specificity
Human HMGB2
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HMGB2 antibody LS-C112165 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGB2 antibody LS-C112165 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HMGB2 antibody LS-C112165 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGB2 antibody LS-C112165 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HMGB2 antibody LS-C112165 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGB2 antibody LS-C112165 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HMGB2 antibody LS-C112165 Western blot of Jurkat lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGB2 antibody LS-C112165 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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