LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
HMG1 / HMGB1 Antibody (Internal, DY650) LS‑C140975
HMGB1 antibody LS-C140975 is a DY650-conjugated rabbit polyclonal antibody to HMGB1 (HMG1) from human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C140975-100
100 µl
Unavailable

Popular HMG1 / HMGB1 Products

HMGB1 Antibody - Staining of HMGB1 in mouse liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Anti-HMGB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4341 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-HMGB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4344 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human, Monkey, Mouse, Rat, Chicken
Applications: Western blot
Anti-HMG1 / HMGB1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8673 dilution 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry

Product Description

HMGB1 antibody LS-C140975 is a DY650-conjugated rabbit polyclonal antibody to HMGB1 (HMG1) from human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
About HMG1 / HMGB1
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. P09429 NM_002128 NP_002119.1

HMGB1 Antibody, Amphoterin Antibody, High mobility group protein B1 Antibody, High mobility group box 1 Antibody, High mobility group protein 1 Antibody, HMG1 Antibody, HMG3 Antibody, SBP-1 Antibody, High-mobility group box 1 Antibody, HMG-1 Antibody


Specifications

Target
Human HMG1 / HMGB1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
DyLight 650. Also available Unconjugated or conjugated with Biotin, DY488, DY550.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:250)
  • Immunofluorescence
  • Western blot (1 µg/ml)
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HMG1 / HMGB1 antibody was raised against a synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]
Epitope
Internal
Specificity
HMGB1 - Oligodendrocyte Marker.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

HMGB1 Antibody - Staining of HMGB1 in mouse liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGB1 Antibody - Staining of HMGB1 in mouse liver. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

HMGB1 Antibody -  Oligodendrocyte Marker - Detectionof HMGB1 (Green) in Hela cells. Nuclei (Blue) are counterstained with Hoecsht 33258.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGB1 Antibody - Oligodendrocyte Marker - Detectionof HMGB1 (Green) in Hela cells. Nuclei (Blue) are counterstained with Hoecsht 33258. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of HMGB1 in whole cell HeLa lysate using LS-C2691. ECL exposure, 1 min.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of HMGB1 in whole cell HeLa lysate using LS-C2691. ECL exposure, 1 min. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Detection of HMGB1 in whole cell HeLa lysate. ECL exposure, 1 min.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of HMGB1 in whole cell HeLa lysate. ECL exposure, 1 min. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

HMGB1 Antibody - Staining of HMGB1 in mouse liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGB1 Antibody - Staining of HMGB1 in mouse liver. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

HMGB1 Antibody -  Oligodendrocyte Marker - Detectionof HMGB1 (Green) in Hela cells. Nuclei (Blue) are counterstained with Hoecsht 33258.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGB1 Antibody - Oligodendrocyte Marker - Detectionof HMGB1 (Green) in Hela cells. Nuclei (Blue) are counterstained with Hoecsht 33258. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of HMGB1 in whole cell HeLa lysate using LS-C2691. ECL exposure, 1 min.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of HMGB1 in whole cell HeLa lysate using LS-C2691. ECL exposure, 1 min. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Detection of HMGB1 in whole cell HeLa lysate. ECL exposure, 1 min.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of HMGB1 in whole cell HeLa lysate. ECL exposure, 1 min. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

HMGB1 Antibody - Staining of HMGB1 in mouse liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGB1 Antibody - Staining of HMGB1 in mouse liver. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

HMGB1 Antibody -  Oligodendrocyte Marker - Detectionof HMGB1 (Green) in Hela cells. Nuclei (Blue) are counterstained with Hoecsht 33258.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGB1 Antibody - Oligodendrocyte Marker - Detectionof HMGB1 (Green) in Hela cells. Nuclei (Blue) are counterstained with Hoecsht 33258. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of HMGB1 in whole cell HeLa lysate using LS-C2691. ECL exposure, 1 min.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of HMGB1 in whole cell HeLa lysate using LS-C2691. ECL exposure, 1 min. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Detection of HMGB1 in whole cell HeLa lysate. ECL exposure, 1 min.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of HMGB1 in whole cell HeLa lysate. ECL exposure, 1 min. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

HMGB1 Antibody - Staining of HMGB1 in mouse liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGB1 Antibody - Staining of HMGB1 in mouse liver. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

HMGB1 Antibody -  Oligodendrocyte Marker - Detectionof HMGB1 (Green) in Hela cells. Nuclei (Blue) are counterstained with Hoecsht 33258.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HMGB1 Antibody - Oligodendrocyte Marker - Detectionof HMGB1 (Green) in Hela cells. Nuclei (Blue) are counterstained with Hoecsht 33258. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of HMGB1 in whole cell HeLa lysate using LS-C2691. ECL exposure, 1 min.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of HMGB1 in whole cell HeLa lysate using LS-C2691. ECL exposure, 1 min. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Detection of HMGB1 in whole cell HeLa lysate. ECL exposure, 1 min.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of HMGB1 in whole cell HeLa lysate. ECL exposure, 1 min. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number