Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone MEM-E/07] (IgG1) to Human HLA-E
Immunofluorescence, Flow Cytometry
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [MEM-E/07]
Protein A purified
Specificity and Use
HLA-E antibody was raised against bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denatured heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working dilution is 2 microgram/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determin
PBS, 15 mM sodium azide, pH 7.4
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.