Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone MEM-E/07] (IgG1) to Human HLA-E
Immunofluorescence, Flow Cytometry
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [MEM-E/07]
Protein A purified
Specificity and Use
HLA-E antibody was raised against bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denatured heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working dilution is 2 microgram/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determin The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, 15 mM sodium azide, pH 7.4
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.