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Anti-HLA-E Antibody (clone 3D12HLA-E) LS-C106800

Catalog Size Price
LS-C106800-25 25 µg Unavailable
LS-C106800-100 100 µg (0.5 mg/ml) Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 3D12HLA-E] (IgG1) to Human HLA-E
Western blot, Immunoprecipitation, Flow Cytometry


Human HLA-E
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [3D12HLA-E]
Affinity purified


  • Western blot
  • Immunoprecipitation
  • Flow Cytometry

  • IHC - Paraffin

Specificity and Use

HLA-E antibody was raised against human HLA-E
This 3D12 (HLA-E) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.


PBS, pH 7.2, <=0.09% sodium azide
Store at 4°C, avoid repeated freeze thaw cycles.
For research use only.

About HLA-E

P13747 NM_005516 NP_005507.3

HLA-E Antibody, EA2.1 Antibody, HLAE Antibody, MHC HLA-E alpha-2.1 Antibody, MHC HLA-E alpha-1 Antibody, EA1.2 Antibody, HLA-6.2 Antibody, MHC class I antigen E Antibody, QA1 Antibody

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons.

Flow Cytometry

Flow Cytometry
Staining of normal human peripheral blood cells with 0.5 ug of Purified Mouse IgG1, K isotype control (open histogram) or 0.5 ug of Purified anti-human HLA-E (3D12HLA-E) (colored histogram) followed by Biotin Anti-Mouse IgG and SAv-PE. Cells in the lymphocyte gate were used for analysis.

Requested From: 
Date Requested: 2/19/2018

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