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| Catalog Number | Size | Price |
|---|---|---|
| LS-B7406-50 | 50 µg (0.5 mg/ml) | $485 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Goat anti‑Human HLA‑DQA2 Antibody (aa40‑50, IHC, WB) LS‑B7406
IHC‑plus™ Polyclonal Goat anti‑Human HLA‑DQA2 Antibody (aa40‑50, IHC, WB) LS‑B7406
Note: This antibody replaces LS-C139657
Antibody:
HLA-DQA2 Goat anti-Human Polyclonal (aa40-50) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Bovine, Goat, Sheep
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HLA-DQA2 Goat anti-Human Polyclonal (aa40-50) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Bovine, Goat, Sheep
Format:
Unconjugated, Unmodified
Specifications
Description
HLA-DQA2 antibody LS-B7406 is an unconjugated goat polyclonal antibody to HLA-DQA2 (aa40-50) from human. It is reactive with human, bovine, goat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HLA-DQA2
Synonyms
HLA-DQA2 | DX-ALPHA | DX alpha chain | MHC class II DQA2 | HLA-DXA
Host
Goat
Reactivity
Human, Bovine, Goat, Sheep
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-QSHGPSGQYTH, from the internal region of the protein sequence according to NP_064440.1.
Epitope
aa40-50
Specificity
Human HLA-DQA2.
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave bands at approx 35kD and 24kD in Human Lymph Node and Tonsil lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 28.0kD according to NP_064440.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HLA-DQA2
Validation
Anti-HLA-DQA2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-HLA-DQA2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ HLA-DQA2 Antibody (aa40-50) for IHC, WB/Western LS-B7406 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Customer Reviews (0)
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Reactivity:
All species
Range:
123.5-10000 pg/ml
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Reactivity:
Range:
4.94-400 pg/ml
Reactivity:
Human
Range:
0.156-10 ng/ml
Source:
Hamster-Chinese
Tag:
His, N-Terminal
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/15/2024
Date Requested: 4/15/2024
