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HLA‑DPA1 Antibody (clone 1E3) LS‑C197415
HLA-DPA1 antibody LS-C197415 is an unconjugated mouse monoclonal antibody to human HLA-DPA1. Validated for ELISA and WB.
Catalog
Size
Price
LS-C197415-100
100 µg
Unavailable

Popular HLA-DPA1 Products

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

HLA-DPA1 antibody LS-C197415 is an unconjugated mouse monoclonal antibody to human HLA-DPA1. Validated for ELISA and WB.
About HLA-DPA1
HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. P20036 X03100 CAA26887.1

HLA-DPA1 Antibody, DP(W4) Antibody, DP(W3) Antibody, HLADP Antibody, HLA-SB alpha chain Antibody, MHC class II DPA1 Antibody, HLASB Antibody, PLT1 Antibody, MHC class II antigen Antibody, MHC class II DP3-alpha Antibody, HLA-DP1A Antibody, MHC class II HLA-DPA1 antigen Antibody


Specifications

Target
Human HLA-DPA1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [1E3]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
HLA-DPA1 antibody was raised against hLA-DPA1 (AAH09956, aa1-260 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human HLA-DPA1
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of HLA-DPA1 expression in transfected 293T cell line by HLA-DPA1 monoclonal antibody (M03), clone 1E3.Lane 1: HLA-DPA1 transfected lysate(29.3 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of HLA-DPA1 expression in transfected 293T cell line by HLA-DPA1 monoclonal antibody (M03), clone 1E3.Lane 1: HLA-DPA1 transfected lysate(29.3 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HLA-DPA1 is approximately 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HLA-DPA1 is approximately 3 ng/ml as a capture antibody.

Western blot

Western Blot analysis of HLA-DPA1 expression in transfected 293T cell line by HLA-DPA1 monoclonal antibody (M03), clone 1E3.Lane 1: HLA-DPA1 transfected lysate(29.3 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of HLA-DPA1 expression in transfected 293T cell line by HLA-DPA1 monoclonal antibody (M03), clone 1E3.Lane 1: HLA-DPA1 transfected lysate(29.3 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HLA-DPA1 is approximately 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HLA-DPA1 is approximately 3 ng/ml as a capture antibody.

Western blot

Western Blot analysis of HLA-DPA1 expression in transfected 293T cell line by HLA-DPA1 monoclonal antibody (M03), clone 1E3.Lane 1: HLA-DPA1 transfected lysate(29.3 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of HLA-DPA1 expression in transfected 293T cell line by HLA-DPA1 monoclonal antibody (M03), clone 1E3.Lane 1: HLA-DPA1 transfected lysate(29.3 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HLA-DPA1 is approximately 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HLA-DPA1 is approximately 3 ng/ml as a capture antibody.

Western blot

Western Blot analysis of HLA-DPA1 expression in transfected 293T cell line by HLA-DPA1 monoclonal antibody (M03), clone 1E3.Lane 1: HLA-DPA1 transfected lysate(29.3 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of HLA-DPA1 expression in transfected 293T cell line by HLA-DPA1 monoclonal antibody (M03), clone 1E3.Lane 1: HLA-DPA1 transfected lysate(29.3 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HLA-DPA1 is approximately 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HLA-DPA1 is approximately 3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/21/2018

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