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Anti-HLA-DMA Antibody (clone 3F12-F11) LS-C133242

Catalog Size Price
LS-C133242-100 100 µg Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 3F12-F11] (IgG1,k) to Human HLA-DMA
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human HLA-DMA
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [3F12-F11]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • IHC - Paraffin (3 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

HLA-DMA antibody was raised against hLA-DMA (AAH11447, 1 a.a. ~ 262 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human HLA-DMA
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About HLA-DMA

P28067 NM_006120 NP_006111.2

HLA-DMA Antibody, DMA Antibody, RING6 Antibody, D6S222E Antibody, HLADM Antibody, MHC class II antigen DMA Antibody

HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages).

Immunohistochemistry

Immunohistochemistry
Immunoperoxidase of monoclonal antibody to HLA-DMA on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 3 ug/ml].

Western blot

Western blot
HLA-DMA monoclonal antibody (M01), clone 3F12-F11 Western blot of HLA-DMA expression in U-2 OS.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged HLA-DMA is approximately 1 ng/ml as a capture antibody.

Requested From: 
Date Requested: 8/22/2017

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