Human HLA-B

Mouse

Human (tested or 100% immunogen sequence identity)

IgM Monoclonal

Unconjugated

Ascites

Unmodified

HLA Class 1Antigen-B17, B35, B44.

Recognizes the human HLA Class 1Antigen-B17, B35, B44. Additional cross-reactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.

• Flow Cytometry

Animal Cell Typing, Tissue Staining, and Chimerism Studies. Please refer to the biotinylated versions of selected antibodies for Flow Cytometry applications. FITC-conjugated and purified versions of these antibodies are available on special order. Directions for Use: For a cytotoxic working dilution, add 0.9 ml of 1% BSA in PBS to 100 ul of10X working stock. Store aliquots at -20C. Expected Values: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.

Lyophilized

100 µl Deionized Water.

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01889 NCBI: NM_005514 NP_005505.2