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Anti-HLA-B Antibody LS-C24479

Catalog Size Price
LS-C24479-100 100 µl Unavailable

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100% Guaranteed
Mouse Monoclonal (IgM) to Human HLA-B
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Details

Human HLA-B
Mouse
Human (tested or 100% immunogen sequence identity)
IgM Monoclonal
Unconjugated
Ascites
Unmodified

Applications

Flow Cytometry

Specificity and Use

HLA-B antibody was raised against hLA Class 1Antigen-B17, B35, B44.
Recognizes the human HLA Class 1Antigen-B17, B35, B44. Additional cross-reactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
Animal Cell Typing, Tissue Staining, and Chimerism Studies. Please refer to the biotinylated versions of selected antibodies for Flow Cytometry applications. FITC-conjugated and purified versions of these antibodies are available on special order. Directions for Use: For a cytotoxic working dilution, add 0.9 ml of 1% BSA in PBS to 100 ul of10X working stock. Store aliquots at -20C. Expected Values: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.

Packaging

Lyophilized.
100 µl Deionized Water.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About HLA-B

P01889 NM_005514 NP_005505.2

HLA-B Antibody, HLAB Antibody, MHC Class I HLA heavy chain Antibody, Leukocyte antigen class I-B Antibody, SPDA1 Antibody

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons.

Requested From: 
Date Requested: 12/18/2017

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