(tested or 100% immunogen sequence identity)
HLA-B antibody was raised against hLA Class 1Antigen-B17, B35, B44.
Recognizes the human HLA Class 1Antigen-B17, B35, B44. Additional cross-reactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
Animal Cell Typing, Tissue Staining, and Chimerism Studies. Please refer to the biotinylated versions of selected antibodies for Flow Cytometry applications. FITC-conjugated and purified versions of these antibodies are available on special order. Directions for Use: For a cytotoxic working dilution, add 0.9 ml of 1% BSA in PBS to 100 ul of10X working stock. Store aliquots at -20C. Expected Values: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
100 µl Deionized Water.
4°C or -20°C, Avoid freeze-thaw cycles.
For research use only.
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