(tested or 100% immunogen sequence identity)
- Flow Cytometry
HLA-B antibody was raised against hLA Class 1 Antigen-B35, B57, B75, B77.
Reacts to HLA Class 1 Antigen-B35, B57, B75, B77. Additional cross-reactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
Suitable for use in Cell Typing, Tissue Staining and Chimerism Studies. For a cytotoxic working dilution, add 0.9 ml of 1% BSA in PBS to 100 ul of10X working dilution. Store aliquots at -20?C. Expected Results: Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
100 µl deionized water
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.
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