Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [BB7.2]
Protein G purified
IHC - Paraffin (10 µg/ml)
IHC - Frozen
Flow Cytometry (10 µg/ml)
Functional Assay (5 - 10 µg/ml)
Specificity and Use
HLA-A2 antibody was raised against papain solubilized HLA-A2.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. The HLA A gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene. The antibody recognizes the human HLA A2 histocompatability antigen. The epitope recognized by this has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. The antibody may be used for the flow cytometric detection of HLA A2 expression and has also been used for immunoaffinity purification of HLA A2 molecules. Functionally this is reported to inhibit MHC restricted cellular cytotoxicity.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Anti-HLA-A2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood lymphocytes with Mouse anti-Human HLA-A2: endotoxin low This image was taken for the unmodified form of this product. Other forms have not been tested.