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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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HLA‑A2 Antibody (clone BB7.2, Low Endotoxin) IHC‑plus™ LS‑B10938
Note: This antibody replaces LS-C188220
HLA-A2 antibody LS-B10938 is an unconjugated mouse monoclonal antibody to human HLA-A2. Validated for Flow, Func, IHC and IP. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B10938-250
250 µg (1 mg/ml)
Unavailable

Popular HLA-A2 Products

Antibody
Species: Human
Applications: Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: Flow Cytometry
Flow Cytometry Image
Species: Human
Applications: IHC, IHC - Paraffin, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: Flow Cytometry

Product Description

HLA-A2 antibody LS-B10938 is an unconjugated mouse monoclonal antibody to human HLA-A2. Validated for Flow, Func, IHC and IP. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human HLA-A2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [BB7.2]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Low Endotoxin
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry (10 µg/ml)
  • Functional Assay (5 - 10 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HLA-A2 antibody was raised against papain solubilized HLA-A2.
Specificity
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. The HLA A gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene. The antibody recognizes the human HLA A2 histocompatability antigen. The epitope recognized by this has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. The antibody may be used for the flow cytometric detection of HLA A2 expression and has also been used for immunoaffinity purification of HLA A2 molecules. Functionally this is reported to inhibit MHC restricted cellular cytotoxicity. The antibody recognizes A2 and A28 others not tested.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-HLA-A2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-HLA-A2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood lymphocytes with Mouse anti-Human HLA-A2: endotoxin low This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood lymphocytes with Mouse anti-Human HLA-A2: endotoxin low This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HLA-A2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-HLA-A2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood lymphocytes with Mouse anti-Human HLA-A2: endotoxin low This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood lymphocytes with Mouse anti-Human HLA-A2: endotoxin low This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HLA-A2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-HLA-A2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood lymphocytes with Mouse anti-Human HLA-A2: endotoxin low This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood lymphocytes with Mouse anti-Human HLA-A2: endotoxin low This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HLA-A2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.
Anti-HLA-A2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood lymphocytes with Mouse anti-Human HLA-A2: endotoxin low This image was taken for the unmodified form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood lymphocytes with Mouse anti-Human HLA-A2: endotoxin low This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 7/17/2018

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