Human HLA-A/B/C

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

MEM-147

Phycoerythrin. Also available Unconjugated.

Size exclusion chromatography

Unmodified

PHA-activated peripheral blood lymphocytes

The antibody MEM-147 reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.

• Western blot
• Immunoprecipitation
• Flow Cytometry (5 µg/ml)
• Applications tested for the base form of this product only

Suggested working dilution is 5 ug/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

PBS, 15mM sodium azide

Store at 2°C to 8°C. Do not freeze. Avoid prolonged exposure to light.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.