Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
HK2 Antibody, Hexokinase II Antibody, Hexokinase type II Antibody, Hexokinase-2, muscle Antibody, HK II Antibody, HXK2 Antibody, Hexokinase-2 Antibody, Muscle form hexokinase Antibody, Hexokinase 2 Antibody, HKII Antibody, Hxk 2 Antibody
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody.
Anti-HK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HK2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.
HEK293T cells transfected with either pCMV6-ENTRY HK2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-HK2 mouse monoclonal, and then analyzed by flow cytometry.