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Anti-HK2 / Hexokinase 2 Antibody (clone 4C5) LS-C114928

Catalog Size Price
LS-C114928-100 100 µl Unavailable

Most Popular HK2 / Hexokinase 2 Antibodies

Anti-HK2 / Hexokinase 2 Antibody (aa74-123) IHC-plus™ LS-B3571
Rabbit Polyclonal (IgG) to Human HK2 / Hexokinase 2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish
IHC, IHC - Paraffin, Western blot
Unconjugated
Anti-HK2 / Hexokinase 2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3571 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HK2 / Hexokinase 2 Antibody (clone 4C5) LS-C114928
Mouse Monoclonal [clone 4C5] (IgG1) to Human HK2 / Hexokinase 2
Human, Dog
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody.
Anti-HK2 / Hexokinase 2 Antibody (N-Terminus) LS-C123747
Rabbit Polyclonal (IgG) to Human HK2 / Hexokinase 2
Human
IHC, Western blot, ELISA
Unconjugated
Anti-HK2 / Hexokinase 2 Antibody LS-C146874
Rabbit Polyclonal to Human HK2 / Hexokinase 2
Human, Mouse
IHC, Western blot, ELISA
Unconjugated
Anti-HK2 / Hexokinase 2 Antibody (clone 3D3) IHC-plus™ LS-B8935
Mouse Monoclonal [clone 3D3] (IgG1) to Human HK2 / Hexokinase 2
Human
IHC, IHC - Paraffin, Flow Cytometry, ELISA
Unconjugated
Anti-HK2 / Hexokinase 2 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

100% Guaranteed
Mouse Monoclonal [clone 4C5] (IgG1) to Human HK2 / Hexokinase 2
Human, Dog
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human HK2 / Hexokinase 2
Mouse
Human, Dog (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [4C5]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:100)

Specificity and Use

Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell.
Human Hexokinase 2 / HK2
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About HK2 / Hexokinase 2

P52789 NM_000189 NP_000180.2

HK2 Antibody, Hexokinase II Antibody, Hexokinase type II Antibody, Hexokinase-2, muscle Antibody, HK II Antibody, HXK2 Antibody, Hexokinase-2 Antibody, Muscle form hexokinase Antibody, Hexokinase 2 Antibody, HKII Antibody, Hxk 2 Antibody

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Immunofluorescence

Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody.
Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody.

Immunofluorescence

Anti-HK2 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HK2.
Anti-HK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HK2.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY HK2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-HK2 mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY HK2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-HK2 mouse monoclonal, and then analyzed by flow cytometry.

Requested From: 
Date Requested: 5/22/2018

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