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Anti-HK2 / Hexokinase 2 Antibody (clone 4C5) LS-C114928

Catalog Size Price
LS-C114928-100 100 µl Unavailable

Most Popular HK2 / Hexokinase 2 Antibodies

Anti-HK2 / Hexokinase 2 Antibody LS-C36657
Rabbit Polyclonal (IgG) to Human HK2 / Hexokinase 2
Human, Monkey
Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-HK2 / Hexokinase 2 Antibody (aa74-123) IHC-plus™ LS-B3571
Rabbit Polyclonal (IgG) to Human HK2 / Hexokinase 2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HK2 / Hexokinase 2 Antibody (clone 4C5) LS-C114928
Mouse Monoclonal [clone 4C5] (IgG1) to Human HK2 / Hexokinase 2
Human, Dog
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunofluorescence Image
Anti-HK2 / Hexokinase 2 Antibody (N-Terminus) LS-C123747
Rabbit Polyclonal (IgG) to Human HK2 / Hexokinase 2
Human
IHC, Western blot, ELISA
Unconjugated
Anti-HK2 / Hexokinase 2 Antibody LS-C146874
Rabbit Polyclonal to Human HK2 / Hexokinase 2
Human, Mouse
IHC, Western blot, ELISA
Unconjugated

100% Guaranteed
Mouse Monoclonal [clone 4C5] (IgG1) to Human HK2 / Hexokinase 2
Human, Dog
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human HK2 / Hexokinase 2
Mouse
Human, Dog (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [4C5]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:100)

Specificity and Use

Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell.
Human Hexokinase 2 / HK2
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About HK2 / Hexokinase 2

P52789 NM_000189 NP_000180.2

HK2 Antibody, Hexokinase II Antibody, Hexokinase type II Antibody, Hexokinase-2, muscle Antibody, HK II Antibody, HXK2 Antibody, Hexokinase-2 Antibody, Muscle form hexokinase Antibody, Hexokinase 2 Antibody, HKII Antibody, Hxk 2 Antibody

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Immunofluorescence

Immunofluorescence
Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody.

Immunofluorescence

Immunofluorescence
Anti-HK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HK2.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either pCMV6-ENTRY HK2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-HK2 mouse monoclonal, and then analyzed by flow cytometry.

Requested From: 
Date Requested: 10/21/2017

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